Helms K
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, Canberra, Australia.
Plant Physiol. 1971 Jun;47(6):799-804. doi: 10.1104/pp.47.6.799.
Hypocotyl collapse in dark-grown seedlings of Phaseolus vulgaris cv. Pinto was due to calcium deficiency. There was no evidence of an associated pathogen. The number of seedlings with hypocotyl collapse decreased and the mean hypocotyl length increased when increasing levels of calcium (0-100 micrograms per gram) were supplied in an external nutrient solution to seedlings grown under sterile conditions.When seedlings were supplied with a complete nutrient solution, containing calcium at 100 micrograms per gram, but minus potassium, magnesium, sulfur, nitrogen, or phosphorus, occasional plants developed hypocotyl collapse symptoms; however, the lengths of hypocotyls varied little from those of controls grown in complete nutrient. When the calcium level in the deficient nutrient solutions was raised to 200 micrograms per gram, the number of plants with hypocotyl collapse was reduced markedly.With complete nutrient solution minus calcium, seedlings developed symptoms of calcium deficiency irrespective of seed size, i.e., irrespective of whether or not the seed contained a total calcium content that was low or relatively high.An increase in hypocotyl length in response to an external supply of calcium was obtained with five cultivars of Phaseolus vulgaris L. and with one of Soja max Piper. A similar response to calcium was obtained for epicotyl growth of a cultivar of Vicia faba L., but not for a cultivar of Pisum sativum L.
菜豆品种平托的黑暗中生长的幼苗下胚轴萎蔫是由缺钙引起的。没有相关病原体的证据。在无菌条件下生长的幼苗，当向外部营养液中添加钙水平增加（0 - 100微克/克）时，下胚轴萎蔫的幼苗数量减少，下胚轴平均长度增加。当给幼苗提供含有100微克/克钙但不含钾、镁、硫、氮或磷的完全营养液时，偶尔有植株出现下胚轴萎蔫症状；然而，下胚轴长度与在完全营养液中生长的对照相比变化不大。当缺乏钙的营养液中的钙水平提高到200微克/克时，下胚轴萎蔫的植株数量明显减少。在不含钙的完全营养液中，幼苗出现缺钙症状，与种子大小无关，即与种子总钙含量低或相对高无关。五个菜豆品种和一个大豆品种对外部供应钙的反应是下胚轴长度增加。蚕豆品种的上胚轴生长对钙有类似反应，但豌豆品种没有。
