Ben-Amotz A, Avron M
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
Plant Physiol. 1972 Feb;49(2):244-8. doi: 10.1104/pp.49.2.244.
The site of action of the inhibitors disalicylidenepropanediamine and pyrophosphate was more closely defined as acting on ferredoxin. Three inhibitors which act on the electron transport path between ferredoxin and NADP: disalicylidenepropanediamine, pyrophosphate, and phosphoadenosinediphosphate ribose, had no effect on photosynthesis in cell free preparations of Dunaliela parva at concentrations which completely inhibited the enzymic activity on which each inhibitor acts. The addition of disalicylidenepropanediamine to dark-grown Euglena gracilis cells prevented the light-induced formation of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, but not of photosynthesis, chlorophyll synthesis, or NAD-dependent glyceraldehyde-3-phosphate dehydrogenase.The above results are interpreted as indicating that, at least under some conditions, a reduced product of photosystem I preceding ferredoxin in the electron transport path can serve as the reductant of CO(2) in photosynthesis.
抑制剂二水杨叉丙二胺和焦磷酸的作用位点被更精确地确定为作用于铁氧化还原蛋白。三种作用于铁氧化还原蛋白和NADP之间电子传递途径的抑制剂：二水杨叉丙二胺、焦磷酸和磷酸腺苷二磷酸核糖，在完全抑制各自所作用的酶活性的浓度下，对杜氏盐藻无细胞制剂中的光合作用没有影响。向黑暗生长的纤细裸藻细胞中添加二水杨叉丙二胺可阻止光诱导的依赖NADP的3-磷酸甘油醛脱氢酶的形成，但不影响光合作用、叶绿素合成或依赖NAD的3-磷酸甘油醛脱氢酶。上述结果被解释为表明，至少在某些条件下，电子传递途径中在铁氧化还原蛋白之前的光系统I的还原产物可以作为光合作用中CO₂的还原剂。
