玉米线粒体分离物中还原型烟酰胺腺嘌呤二核苷酸磷酸的氧化。
Oxidation of reduced nicotinamide adenine dinucleotide phosphate by isolated corn mitochondria.
机构信息
Department of Agronomy, University of Illinois, Urbana, Illinois 61801.
出版信息
Plant Physiol. 1972 Mar;49(3):353-7. doi: 10.1104/pp.49.3.353.
Abstract
Isolated corn (Zea mays L.) mitochondria were found to oxidize reduced nicotinamide adenine dinucleotide phosphate in a KCl reaction medium. This oxidation was dependent on the presence of calcium or phosphate or both. Strontium and manganese substituted for calcium, but magnesium or barium did not. The oxidation of NADPH produced contraction of mitochondria swollen in KCl. Further evidence that the oxidation of NADPH was coupled was observed in respiratory control and adenosine diphosphate-oxygen ratios that were comparable to those reported for reduced nicotinamide adenine dinucleotide. The pathways of electron flow from NADH and NADPH were compared through the addition of electron transport inhibitors. The only difference between the two dinucleotides was that amytal was found to inhibit almost totally the state 3 oxidation of NADPH, but had little effect on the state 3 oxidation of NADH. The hypothetical pathways for electron flow from NADPH are discussed, as are the possible sites of calcium and phosphate stimulation.
摘要
分离的玉米（Zea mays L.）线粒体在 KCl 反应介质中被发现可氧化还原型烟酰胺腺嘌呤二核苷酸磷酸。这种氧化作用依赖于钙或磷酸盐或两者的存在。锶和锰取代钙，但镁或钡则不能。NADPH 的氧化导致在 KCl 中膨胀的线粒体收缩。在呼吸控制和腺苷二磷酸-氧比中观察到 NADPH 氧化偶联的进一步证据，这些比值与报道的还原型烟酰胺腺嘌呤二核苷酸相当。通过添加电子传递抑制剂比较了 NADH 和 NADPH 的电子流途径。这两种二核苷酸之间的唯一区别是，戊巴比妥被发现几乎完全抑制 NADPH 的状态 3 氧化，但对 NADH 的状态 3 氧化几乎没有影响。讨论了电子从 NADPH 流动的假设途径，以及钙和磷酸盐刺激的可能部位。
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