VANDEMARK P J, SMITH P F
J Bacteriol. 1964 Jul;88(1):122-9. doi: 10.1128/jb.88.1.122-129.1964.
VanDemark, P. J. (University of South Dakota, Vermillion), and P. F. Smith. Respiratory pathways in the Mycoplasma. II. Pathway of electron transport during oxidation of reduced nicotinamide adenine dinucleotide by Mycoplasma hominis. J. Bacteriol. 88:122-129. 1964.-Unlike the flavin-terminated respiratory pathway of the fermentative Mycoplasma, the respiratory chain of the nonfermentative M. hominis strain 07 appears to be more complex, involving quinones and cytochromes in addition to flavins. In addition to reduction by reduced nicotine adenine dinucleotide (NADH) and reduced nicotine adenine dinucleotide phosphate, nonpyridine nucleotide-linked reduction of the respiratory chain of this organism occurred with succinate, lactate, and short-chained acyl coenzyme A derivatives as electron donors. Enzymes catalyzing the oxidation of NADH included an NADH oxidase, a diaphorase, a quinone reductase, and a cytochrome c reductase. The oxidation of NADH was sensitive to a variety of inhibitors, including 10(-4)m Atabrine, 10(-3)m sodium amytal, 10(-5)mp-chloromercuribenzoate, 10(-4)m antimycin A, and 10(-4)m potassium cyanide. The oxidase was resolved by the addition of 5% trichloroacetic acid and reactivated by the addition of flavin adenine dinucleotide but not flavin mononucleotide. The M. hominis sonic extract contained an NADH-coenzyme Q reductase. The oxidation of NADH was stimulated by the addition of either menadione or vitamin K(2) (C(35)). The oxidase was inactivated by extraction with ether or irradiation at 360 mmu. The ether-inactivated enzyme was partially reactivated by the addition of "lipid" extract of the enzyme and coenzyme Q(6). Difference spectra of the cell extracts revealed the presence of "b" and "a" type cytochromes. These cell extracts were found to contain a cyanide-and azide-sensitive cytochrome oxidase and catalase.
范德马克，P. J.（南达科他大学，弗米利恩）和P. F. 史密斯。支原体中的呼吸途径。II. 人型支原体氧化还原型烟酰胺腺嘌呤二核苷酸过程中的电子传递途径。《细菌学杂志》88:122 - 129。1964年。——与发酵性支原体以黄素终止的呼吸途径不同，非发酵性人型支原体菌株07的呼吸链似乎更为复杂，除黄素外还涉及醌类和细胞色素。除了被还原型烟酰胺腺嘌呤二核苷酸（NADH）和还原型烟酰胺腺嘌呤二核苷酸磷酸还原外，该生物体呼吸链的非吡啶核苷酸连接还原反应还发生在琥珀酸、乳酸以及短链酰基辅酶A衍生物作为电子供体的情况下。催化NADH氧化的酶包括一种NADH氧化酶、一种递氢酶、一种醌还原酶和一种细胞色素c还原酶。NADH的氧化对多种抑制剂敏感，包括10⁻⁴m阿的平、10⁻³m戊巴比妥钠、10⁻⁵m对氯汞苯甲酸、10⁻⁴m抗霉素A和10⁻⁴m氰化钾。加入5%三氯乙酸可使氧化酶沉淀，加入黄素腺嘌呤二核苷酸可使其重新激活，但黄素单核苷酸则不能。人型支原体超声提取物含有一种NADH - 辅酶Q还原酶。加入甲萘醌或维生素K₂（C₃₅）可刺激NADH的氧化。用乙醚提取或在360毫微米处照射可使氧化酶失活。加入酶的“脂质”提取物和辅酶Q₆可使经乙醚失活的酶部分重新激活。细胞提取物的差示光谱显示存在“b”型和“a”型细胞色素。发现这些细胞提取物含有一种对氰化物和叠氮化物敏感的细胞色素氧化酶和过氧化氢酶。
