Department of Biochemistry, University of California, Riverside, California 92502.
Plant Physiol. 1972 Apr;49(4):542-5. doi: 10.1104/pp.49.4.542.
Coleoptile sections of Avena sativa L. were pretreated with sodium fluoride or peroxyacetyl nitrate at levels which inhibit auxin-induced growth but did not affect glucose uptake or CO(2) production when postincubated for 30 minutes in a (14)C-glucose medium without auxin. Labeling of metabolites involved in cell wall synthesis was measured. Peroxyacetyl nitrate decreased labeling, and it was concluded that the pool size of uridine di-phosphoglucose, sucrose, and cell wall polysaccharides decreased compared to control. The changes suggest that peroxyacetyl nitrate inactivated sucrose and cell wall synthesizing enzymes including cellulose synthetase and decreased cell growth by inhibiting production of cell wall constituents. Fluoride treatment had no effect on production of cell wall polysaccharides, with or without indoleacetic acid stimulation of growth. The only change after fluoride treatment was a decrease in uridine diphosphoglucose during incubation without indoleacetic acid, a decrease that disappeared when indoleacetic acid was present. It was concluded that some other aspect of cell wall metabolism, not determined here, was involved in fluoride-induced inhibition of growth.
对燕麦胚芽鞘进行预处理，使用的氟化物或过氧乙酰硝酸盐的浓度可以抑制生长素诱导的生长，但不会影响在没有生长素的（14）C-葡萄糖培养基中孵育 30 分钟后的葡萄糖摄取或 CO2 产生。测量参与细胞壁合成的代谢物的标记。过氧乙酰硝酸盐降低了标记，因此可以得出结论，与对照相比，尿苷二磷酸葡萄糖、蔗糖和细胞壁多糖的池大小减少。这些变化表明，过氧乙酰硝酸盐使蔗糖和细胞壁合成酶（包括纤维素合成酶）失活，并通过抑制细胞壁成分的产生来减少细胞生长。氟化物处理对细胞壁多糖的产生没有影响，无论是否有生长素刺激生长。氟化物处理后的唯一变化是在没有生长素的情况下孵育时尿苷二磷酸葡萄糖减少，当存在生长素时这种减少消失。因此，可以得出结论，在氟化物诱导的生长抑制中涉及细胞壁代谢的某些其他方面，这里尚未确定。
