Evans M L, Ray P M
J Gen Physiol. 1969 Jan;53(1):1-20. doi: 10.1085/jgp.53.1.1.
The timing of the auxin response was followed in oat and corn coleoptile tissue by a sensitive optical method in which the elongation of about a dozen coleoptile segments was recorded automatically. The response possesses a latent period of about 10 min at 23 degrees C, which is extended by low concentrations of KCN or by reducing the temperature, but is not extended by pretreatments with actinomycin D, puromycin, or cycloheximide at concentrations that partially inhibit the elongation response. Analysis of the data indicates that auxin probably does not act on the elongation of these tissues by promoting the synthesis of informational RNA or of enzymatic protein. Not excluded is the possibility that auxin acts at the translational level to induce synthesis of a structural protein, such as cell wall protein or membrane protein. While the data do not provide direct support for this hypothesis, the speed with which cycloheximide inhibits elongation suggests that continual protein synthesis may be important in the mechanism of cell wall expansion.
通过一种灵敏的光学方法，跟踪燕麦和玉米胚芽鞘组织中生长素反应的时间，该方法可自动记录约十二个胚芽鞘切段的伸长情况。在23摄氏度时，该反应具有约10分钟的潜伏期，低浓度的氰化钾或降低温度会延长潜伏期，但在部分抑制伸长反应的浓度下，用放线菌素D、嘌呤霉素或环己酰亚胺预处理不会延长潜伏期。数据分析表明，生长素可能不是通过促进信息RNA或酶蛋白的合成来作用于这些组织的伸长。生长素在翻译水平上作用以诱导结构蛋白（如细胞壁蛋白或膜蛋白）合成的可能性并未排除。虽然数据并未直接支持这一假设，但环己酰亚胺抑制伸长的速度表明，持续的蛋白质合成可能在细胞壁扩张机制中很重要。
