栅藻末端氧化酶。
Terminal Oxidases of Chlorella pyrenoidosa.
机构信息
Department of Biophysics, University of Western Ontario, London, Canada.
出版信息
Plant Physiol. 1972 May;49(5):775-8. doi: 10.1104/pp.49.5.775.
In studies of the kinetics of oxygen uptake by glucose-stimulated Chlorella pyrenoidosa, two terminal oxidases could be distinguished. The cytochrome oxidase of Chlorella has a Km (O(2)) of 2.1 +/- 0.3 mum, while the second oxidase has a Km (O(2)) of 6.7 +/- 0.5 mum, and a maximum capacity about one-quarter of that of the cytochrome system. The identity of the second oxidase is unknown, but it is not inhibited by carbon monoxide, 1 mm cyanide, 0.1 mm thiocyanate, or 1 mm 8-hydroxyquinoline. In fresh cultures, the second oxidase accounts for at most 35% of the total oxygen uptake.
在研究葡萄糖刺激的蛋白核小球藻耗氧动力学的过程中，我们可以区分两种末端氧化酶。小球藻细胞色素氧化酶的 Km（O2）为 2.1±0.3 μM，而第二种氧化酶的 Km（O2）为 6.7±0.5 μM，其最大容量约为细胞色素系统的四分之一。第二种氧化酶的身份尚不清楚，但它不受一氧化碳、1mm 氰化物、0.1mm 硫氰酸盐或 1mm 8-羟基喹啉的抑制。在新鲜培养物中，第二种氧化酶最多占总耗氧量的 35%。
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