菲力平对用不同类型甾体制备的脂质体的影响。
Effect of filipin on liposomes prepared with different types of steroids.
机构信息
Department of Agricultural Chemistry, Washington State University, Pullman, Washington 99163.
出版信息
Plant Physiol. 1972 May;49(5):852-6. doi: 10.1104/pp.49.5.852.
The action of the polyene antibiotic filipin on the permeability of liposomes prepared with lecithin and several plant and other steroids was studied. The effect of filipin was found to be dependent upon the type of steroid incorporated into the membrane. The interaction of filipin with steroids was found to be related both to the functional group at the 3-position and the aliphatic chain of the steroid. Complex formation of the steroid with filipin in aqueous solutions, as detected by ultraviolet spectrophotometry, correlated with the ability of filipin to increase the permeability of the liposomes.From these results it was suggested that the action of filipin could be used as a diagnostic tool to determine the presence of different steroids in plant membranes. Differences in the sensitivity of subcellular organelles to filipin may be due to differences in the steroid composition of their membranes.
研究了多烯抗生素 filipin 对由卵磷脂和几种植物固醇及其他固醇制备的脂质体通透性的作用。发现 filipin 的作用取决于掺入膜中的固醇的类型。发现 filipin 与固醇的相互作用既与 3 位的官能团有关，也与固醇的脂肪链有关。甾体与 filipin 在水溶液中的络合作用，通过紫外分光光度法检测到，与 filipin 增加脂质体通透性的能力相关。根据这些结果，有人认为 filipin 的作用可以用作诊断工具，以确定植物膜中存在不同的固醇。细胞器官对 filipin 的敏感性的差异可能是由于它们的膜中固醇组成的差异。
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