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从眼点藻(Euglena gracilis var. bacillaris)中大规模分离和纯化眼点颗粒。

Large Scale Isolation and Purification of Eyespot Granules from Euglena gracilis var. bacillaris.

机构信息

Department of Botany, University of Tennessee, Knoxville, Tennessee 37916.

出版信息

Plant Physiol. 1972 Jun;49(6):881-5. doi: 10.1104/pp.49.6.881.

DOI:10.1104/pp.49.6.881
PMID:16658076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC366072/
Abstract

Large volumes of eyespot granules were isolated from homogenates of Euglena gracilis Klebs var. bacillaris Pringsheim by flotation centrifugation in a Beckman Ti-15 zonal rotor, and were further purified by centrifugation in a swinging bucket rotor. Examination with the electron microscope showed the eyespot granules to be free from other cellular material. Freezing had no apparent effect on the structure or on the absorption properties of the eyespots. Absorption spectra of pure fractions of eyespot granules free of chloroplast contamination showed the previously reported curves in the range of 360 to 520 nanometers, as well as a peak at 660 to 675 nanometers. The procedure for the large scale isolation of eyespot granules from Euglena gracilis is compared with other methods which have employed conventional centrifugation, and the significance of the use of zonal rotors for isolating large quantities of pure eyespot granules is discussed.

摘要

大量的眼点颗粒从 Euglena gracilis Klebs var. bacillaris Pringsheim 的匀浆中通过在 Beckman Ti-15 区带转子中的浮选离心分离出来，并通过在摆动桶转子中的离心进一步纯化。电子显微镜检查显示眼点颗粒没有其他细胞物质。冷冻对眼点的结构或吸收特性没有明显影响。没有叶绿体污染的纯眼点颗粒的吸收光谱显示了之前在 360 至 520 纳米范围内报告的曲线，以及在 660 至 675 纳米处的峰。从 Euglena gracilis 中大规模分离眼点颗粒的程序与其他使用常规离心的方法进行了比较，并讨论了使用区带转子分离大量纯眼点颗粒的意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51e3/366072/6e4927e241f6/plntphys00242-0016-c.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51e3/366072/aa10b60e0e83/plntphys00242-0016-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51e3/366072/3c5f5d33c7c2/plntphys00242-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51e3/366072/688caa55144f/plntphys00242-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51e3/366072/dd11366fc68e/plntphys00242-0015-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51e3/366072/8ab8ac1293d8/plntphys00242-0015-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51e3/366072/aa10b60e0e83/plntphys00242-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51e3/366072/d6f3eb13e405/plntphys00242-0016-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51e3/366072/6e4927e241f6/plntphys00242-0016-c.jpg

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本文引用的文献

1
PHOTOTAXIS IN EUGLENA. I. ISOLATION OF THE EYE-SPOT GRANULES AND IDENTIFICATION OF THE EYE-SPOT PIGMENTS.眼虫的趋光性。一、眼点颗粒的分离及眼点色素的鉴定
Biochim Biophys Acta. 1964 Mar 30;79:371-8. doi: 10.1016/0926-6585(64)90017-2.
2
PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY.用于电子显微镜检查的塑料包埋混合物
Stain Technol. 1964 Mar;39:111-4.
3
The isolation of oral structures from Tetrahymena pyriformis by low-speed zonal centrifugation.通过低速区带离心法从梨形四膜虫中分离口腔结构。
眼虫叶绿体早期发育相关事件：7. 除草剂 SAN 9789（4-氯-5-（甲氨基）-2-（α，α，α-三氟-间-甲苯基）-3-（2H）哒嗪酮）对类胡萝卜素生物合成的抑制及其发育后果。
Plant Physiol. 1976 Feb;57(2):260-9. doi: 10.1104/pp.57.2.260.
4
Light Antennas in phototactic algae.趋光藻类中的光天线
Microbiol Rev. 1980 Dec;44(4):572-630. doi: 10.1128/mr.44.4.572-630.1980.
5
Effects of Mg2+ and Ca2+ on photoinduced Euglena flagellar responses.镁离子和钙离子对光诱导的眼虫鞭毛反应的影响。
J Cell Biol. 1980 Feb;84(2):355-63. doi: 10.1083/jcb.84.2.355.
6
Spectroscopic properties and related functions of the stigma measured in living cells of Euglena gracilis.在纤细裸藻活细胞中测量的柱头的光谱特性及相关功能。
Arch Microbiol. 1976 Dec 1;111(1-2):73-6. doi: 10.1007/BF00446551.
7
Separation and identification of the carotenoid pigments of stigmata isolated from light-grown cells of Euglena gracilis strain Z.从纤细裸藻Z株光照培养细胞中分离得到的柱头类胡萝卜素色素的分离与鉴定。
Arch Microbiol. 1979 Jun;121(3):207-11. doi: 10.1007/BF00425057.
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Molecular sensory physiology of Euglena.眼虫的分子感觉生理学
Naturwissenschaften. 1976 Sep;63(9):412-7. doi: 10.1007/BF00599409.
Natl Cancer Inst Monogr. 1966 Jun;21:317-21.
4
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