Botanisches Institut der Universität München and Fachbereich Biologie der Universität Regensburg, 8400 Regensburg, Universitätsstrasse 31-Postfach, Germany.
Plant Physiol. 1972 Jun;49(6):904-6. doi: 10.1104/pp.49.6.904.
Light-dependent formation of ribulose-1,5 diphosphate is completely inhibited by low concentrations of 3-(3,4-dichlorophenyl)-1,1-dimethylurea which do not severely affect cyclic photophosphorylation. Also in Scenedesmus mutant number 11, capable of cyclic photophosphorylation, cellular ribulose-1,5 diphosphate-levels do not increase upon illumination. When mutant cells are H(2) adapted, however, a light-dependent formation of ribulose-1,5 diphosphate is observed in the presence of H(2). From these results it has been concluded that at least part of the Calvin cycle does not operate in the dark, since a reductant is lacking which is generated in the light.
在低浓度的 3-(3,4-二氯苯基)-1,1-二甲基脲的作用下,光依赖性核酮糖-1,5-二磷酸的形成完全被抑制,而这种浓度的 3-(3,4-二氯苯基)-1,1-二甲基脲并不会严重影响环式光合磷酸化。同样,在能够进行环式光合磷酸化的 Scenedesmus 突变体 11 中,细胞内核酮糖-1,5-二磷酸的水平在光照下也不会增加。然而,当突变体细胞适应氢气时,在氢气存在的情况下,会观察到光依赖性核酮糖-1,5-二磷酸的形成。根据这些结果可以得出结论,至少部分卡尔文循环在黑暗中无法运行,因为缺乏在光照下生成的还原剂。