Feung C S, Hamilton R H, Witham F H, Mumma R O
Departments of Biology and Entomology, The Pennsylvania State University, University Park, Pennsylvania 16802.
Plant Physiol. 1972 Jul;50(1):80-6. doi: 10.1104/pp.50.1.80.
Soybean (Glycine max L.) cotyledon callus grown on radioactive 2,4-dichlorophenoxyacetic acid (2,4-D-1-(14)C) as an auxin produced 2,4-D metabolites, which qualitatively and quantitatively changed with time. Water soluble fractions from the tissue exhibited a steady increase in radioactivity during the course of 24 days. Following beta-glucosidase treatment, at least eight aglycones were obtained from the water soluble fraction of the tissue after 8 days. The metabolite, 4-hydroxy-2,5-dichlorophenoxyacetic acid was the most abundant aglycone during the entire 32 day growth period while 4-hydroxy-2,3-dichlorophenoxyacetic acid was detected as a minor metabolite. Radioactivity in the ether soluble acidic fractions reached a maximum of 82% of the total in the tissue after 2 days. The level then decreased to 44% by the end of 24 days. A total of seven ether soluble components were detected. In addition to 2,4-D glutamic acid, which was detected in high amounts after 24 hours, 2,4-D aspartic acid was found to be the most abundant ether soluble metabolite after longer time periods. Mass spectral data and a fragmentation pattern are presented for 2,4-D aspartic acid.
以放射性2,4-二氯苯氧乙酸（2,4-D-1-(14)C）作为生长素培养的大豆（Glycine max L.）子叶愈伤组织产生了2,4-D代谢物，其在质量和数量上均随时间变化。在24天的培养过程中，组织的水溶性部分放射性稳步增加。经β-葡萄糖苷酶处理后，8天后从组织的水溶性部分获得了至少8种苷元。在整个32天的生长期间，代谢物4-羟基-2,5-二氯苯氧乙酸是最丰富的苷元，而4-羟基-2,3-二氯苯氧乙酸被检测为次要代谢物。醚溶性酸性部分的放射性在2天后达到组织中总放射性的82%的最大值。然后该水平在24天结束时降至44%。总共检测到7种醚溶性成分。除了在24小时后大量检测到的2,4-D谷氨酸外，2,4-D天冬氨酸被发现是较长时间后最丰富的醚溶性代谢物。给出了2,4-D天冬氨酸质谱数据和裂解模式。
