十六烷基三甲基溴化铵对马铃薯块茎颗粒态淀粉合成酶活性的影响。
Effect of cetyltrimethylammonium bromide on the activity of particulate starch synthetase from potato tuber.
机构信息
Instituto de Investigaciones Bioquímicas "Fundación Campomar" and Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires 28, Argentina.
出版信息
Plant Physiol. 1972 Aug;50(2):205-7. doi: 10.1104/pp.50.2.205.
Abstract
The action of some detergents on the incorporation of glucose from uridine diphosphate glucose or adenosine diphosphate glucose into the potato tuber starch grain was studied. It was found that the cationic detergent, cetyltrimethylammonium bromide, produces a rapid binding of both sugar nucleotides to the grain and a great increase in the incorporation of glucose into the polysaccharide. Kinetic constants of starch synthetase are also modified, there being an affinity increase for both sugar nucleotides. Neutral detergents are without effect and anionic detergents are inhibitors.
摘要
研究了一些清洁剂对葡萄糖从尿苷二磷酸葡萄糖或腺苷二磷酸葡萄糖掺入马铃薯淀粉粒的作用。结果发现，阳离子清洁剂十六烷基三甲基溴化铵能使两种糖核苷酸迅速与颗粒结合，并大大增加葡萄糖掺入多糖中。淀粉合成酶的动力学常数也发生了变化，对两种糖核苷酸的亲和力都增加了。中性清洁剂没有效果，阴离子清洁剂是抑制剂。
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