菜豆 l-半胱氨酸生物合成酶的研究
Studies of l-Cysteine Biosynthetic Enzymes in Phaseolus vulgaris L.
机构信息
Department of Botany, Ohio University, Athens, Ohio 45701.
出版信息
Plant Physiol. 1972 Oct;50(4):477-9. doi: 10.1104/pp.50.4.477.
Abstract
In higher plants the biosynthesis of l-cysteine from l-serine, acetylCoA, and sulfide requires serine transacetylase and O-acetylserine sulfhydrylase. The distribution of these enzymes in kidney bean (Phaseolus vulgaris L. cv. Red Kidney) seedlings was determined. Between one-third and two-thirds of the serine transacetylase activity was associated with mitochondria, whereas all of the O-acetyl-serine sulfhydrylase activity was present in the soluble fraction of cell homogenates. In a 14-day plant approximately two-thirds of the O-acetylserine sulfhydrylase activity and approximately one-half of the serine transacetylase activity was found in the leaves.Sulfur-deficient plants were grown to determine the effect of sulfur status on the levels of cysteine biosynthetic enzymes. Total extractable serine transacetylase activity was not affected by sulfur deficiency; in contrast, there was an increase in O-acetylserine sulfhydrylase activity under these conditions.
摘要
在高等植物中，从 l-丝氨酸、乙酰辅酶 A 和硫化物生物合成 l-半胱氨酸需要丝氨酸乙酰转移酶和 O-乙酰丝氨酸巯基酶。本文测定了这些酶在菜豆（Phaseolus vulgaris L. cv. Red Kidney）幼苗中的分布。三分之一到三分之二的丝氨酸乙酰转移酶活性与线粒体相关，而所有的 O-乙酰丝氨酸巯基酶活性都存在于细胞匀浆的可溶性部分。在 14 天的植物中，约三分之二的 O-乙酰丝氨酸巯基酶活性和约一半的丝氨酸乙酰转移酶活性存在于叶片中。硫缺乏的植物被生长以确定硫状态对半胱氨酸生物合成酶水平的影响。总可提取的丝氨酸乙酰转移酶活性不受硫缺乏的影响；相比之下，在这些条件下 O-乙酰丝氨酸巯基酶活性增加。
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