Mitchell R C, Villiers T A
Department of Plant Biology, University of Natal, Durban, South Africa.
Plant Physiol. 1972 Dec;50(6):671-4. doi: 10.1104/pp.50.6.671.
Lettuce (Lactuca sativa) seeds var. Grand Rapids could be maintained many weeks in the dark without germination. Following illumination with white light, a gradual increase in polyribosome population up to the time of germination was demonstrated by sucrose gradient centrifugation. Polysomes could not be detected in imbibed seeds maintained continuously in the dark. Thus, polysome formation and therefore the capacity for a high rate of protein synthesis required for germination and growth, is not associated with the process of imbibition, but is dependent upon the seeds having received the dormancy-breaking stimulus of illumination.
大急流城生菜（Lactuca sativa）品种的种子可以在黑暗中保存数周而不发芽。用白光照射后，通过蔗糖梯度离心法证明，在发芽前多核糖体数量逐渐增加。在持续黑暗中浸泡的种子中未检测到多聚核糖体。因此，多聚核糖体的形成以及发芽和生长所需的高速率蛋白质合成能力，与吸水过程无关，而是取决于种子是否受到了打破休眠的光照刺激。
