从蛋白核小球藻中部分纯化 NADH-硝酸还原酶复合物。
Partial Purification of the NADH-Nitrate Reductase Complex from Chlorella pyrenoidosa.
机构信息
Department of Biology, University of Virginia, Charlottesville, Virginia 22903.
出版信息
Plant Physiol. 1973 Mar;51(3):591-3. doi: 10.1104/pp.51.3.591.
Abstract
The nitrate reductase complex from Chlorella pyrenoidosa has been purified by a procedure which includes as main steps, ammonium sulfate fractionation, polyethylene glycol treatment, and DEAE-cellulose chromatography. The Michaelis constants for NADH, FAD, and NO(3) (-) in the NADH-nitrate reductase assay are 10 mum, 2.6 mum, and 0.23 mm, respectively. Heat treatment exerts varying effects on the enzymatic activities associated with the nitrate reductase complex.
摘要
从蛋白核小球藻中纯化的硝酸还原酶复合物，通过包括以下主要步骤的程序进行：硫酸铵分级沉淀、聚乙二醇处理和 DEAE-纤维素层析。在 NADH-硝酸还原酶测定中，NADH、FAD 和 NO₃⁻的米氏常数分别为 10 µm、2.6 µm 和 0.23 mM。热处理对与硝酸还原酶复合物相关的酶活性有不同的影响。
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