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有机溶剂浸泡促进山茶花花粉生长。

Acceleration of the Growth of Camellia sasanqua Pollen by Soaking in Organic Solvent.

机构信息

Biological Institute, Yokohama City University, Kanazawa-ku, Yokohama 236, Japan.

出版信息

Plant Physiol. 1973 Nov;52(5):508-9. doi: 10.1104/pp.52.5.508.

DOI:10.1104/pp.52.5.508
PMID:16658593
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC366533/
Abstract

Camellia sasanqua pollen that had been soaked in acetone or diethyl ether for only 5 minutes grew three to four times longer pollen tubes than unsoaked pollen. Although the acceleration of pollen tube growth was observed when the pollen had been soaked in cold solvents (5 C, -15 C) for 100 days, soaking in warm solvents (30 C, 24 C) caused it to decrease gradually, and the pollen eventually lost the ability to germinate. The acceleration may be caused by removing inhibitor from the pollen grain by the organic solvents.

摘要

山茶花花粉在丙酮或乙醚中浸泡仅 5 分钟,花粉管的生长长度就会增加三到四倍,而未浸泡的花粉则没有明显变化。尽管当花粉在冷溶剂(5°C、-15°C)中浸泡 100 天时观察到花粉管生长的加速,但在温暖溶剂(30°C、24°C)中浸泡会导致其逐渐减少,花粉最终丧失发芽能力。这种加速可能是有机溶剂从花粉粒中去除抑制剂引起的。

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引用本文的文献

1
Mitochondrial development and activity of binucleate and trinucleate pollen during germination in vitro.双核和三核花粉在体外萌发过程中线粒体的发育和活性。
Planta. 1979 Jan;145(1):25-36. doi: 10.1007/BF00379924.

本文引用的文献

1
Storage in an organic solvent as a means for preserving viability of pollen grains.将花粉粒保存在有机溶剂中作为一种保持其活力的方法。
Stain Technol. 1972 May;47(3):137-9. doi: 10.3109/10520297209116468.
2
Retaining the viability of the resting egg of brine-shrimp (Artemia salina) in organic solvent.保持卤虫(卤虫)休眠卵在有机溶剂中的活力。
Exp Cell Res. 1973 Apr;78(2):470-1. doi: 10.1016/0014-4827(73)90095-5.