Department of Biology, Brandeis University, Waltham, Massachusetts 02154.
Plant Physiol. 1973 Dec;52(6):674-6. doi: 10.1104/pp.52.6.674.
A triose phosphate/3-phosphoglycerate shuttle for the indirect transfer of photosynthetically reduced NADP from chloroplasts to the cytoplasm has been demonstrated in vitro. Triose phosphate, formed from 3-phosphoglycerate in the chloroplast, was oxidized back to 3-phosphoglycerate outside the chloroplast by the nonreversible d-glyceraldehyde 3-phosphate dehydrogenase reaction which is specific for NADP. The 3-phosphoglycerate could presumably return to the chloroplast to complete the shuttle. The properties of nonreversible d-glyceraldehyde 3-phosphate dehydrogenase are considered particularly suitable for effective operation of this shuttle system.
已在体外证明,三碳糖磷酸/3-磷酸甘油酸穿梭系统可将光合作用还原的 NADP 从叶绿体间接转移到细胞质中。在叶绿体中由 3-磷酸甘油酸形成的三碳糖磷酸,可通过非可逆的 d-甘油醛 3-磷酸脱氢酶反应氧化回叶绿体外的 3-磷酸甘油酸,该反应特异性地针对 NADP。3-磷酸甘油酸可能会返回叶绿体以完成穿梭。非可逆的 d-甘油醛 3-磷酸脱氢酶的特性被认为特别适合这种穿梭系统的有效运作。