Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824.
Plant Physiol. 1974 Dec;54(6):826-8. doi: 10.1104/pp.54.6.826.
When excised embryos of Agrostemma githago were incubated with nitrate, the activities of both nitrate reductase and nitrite reductase were enhanced. By contrast, benzyladenine induced nitrate reductase only. Our data suggest that nitrate affected nitrite reductase activity directly, without first being reduced to nitrite. When the endogenous nitrite production was increased by raising the level of nitrate reductase through simultaneous treatment with nitrate and benzyladenine, the activity of nitrite reductase was not higher than in embryos treated with nitrate alone. On the other hand, tungstate given together with nitrate drastically inhibited the development of nitrate reductase activity without reducing the enhancement of nitrite reductase activity. Nitrite enhanced nitrite reductase activity, though less efficiently than nitrate.
当荠菜胚胎用硝酸盐孵育时，硝酸还原酶和亚硝酸还原酶的活性都增强了。相比之下，苄基腺嘌呤只诱导硝酸还原酶。我们的数据表明，硝酸盐直接影响亚硝酸还原酶的活性，而不需要先还原为亚硝酸盐。当通过同时用硝酸盐和苄基腺嘌呤处理来提高硝酸还原酶的水平从而增加内源性亚硝酸盐的产生时，亚硝酸还原酶的活性并不高于单独用硝酸盐处理的胚胎。另一方面，钨酸盐与硝酸盐一起使用会严重抑制硝酸还原酶活性的发展，而不会降低亚硝酸还原酶活性的增强。亚硝酸盐增强了亚硝酸还原酶的活性，但其效率不如硝酸盐。
