Department of Plant Sciences, Indiana University, Bloomington, Indiana 47401.
Plant Physiol. 1975 Feb;55(2):183-6. doi: 10.1104/pp.55.2.183.
The cell structure of Chlamydomonas reinhardi is disrupted by brief exposure to sonication. The extent of cell breakage can be determined quickly by cell count with the light microscope. Rates of photochemical activities of briefly sonicated cells approach those reported for higher plant chloroplasts. These activities are a sensitive function of time of sonication and sonic power used. The method of brief sonication is rapid and convenient and gives a stable preparation useful for determining photochemical activities in Chlamydomonas.
短暂超声处理会破坏莱茵衣藻的细胞结构。用光学显微镜通过细胞计数可以快速确定细胞破碎程度。短暂超声处理的细胞的光化学活性速率接近已报道的高等植物叶绿体的光化学活性速率。这些活性是超声时间和超声功率的敏感函数。短暂超声处理方法快速便捷,可获得稳定的制备物,可用于测定莱茵衣藻的光化学活性。