Givan A L, Levine R P
Plant Physiol. 1967 Sep;42(9):1264-8. doi: 10.1104/pp.42.9.1264.
Electron transport activity and absorbance changes associated with P700 were investigated in a mutant strain of Chlamydomonas reinhardi with impaired photosynthesis. This mutant strain, ac-8oa, cannot reduce NADP with electrons from either water or dye and ascorbate, but it has considerable Hill activity. The mutant strain shows none of the absorbance changes characteristic of P700. Although unable to carry out cyclic photosynthetic phosphorylation, ac-8oa is able to synthesize ATP when ferricyanide is provided as an electron acceptor. These observations lead to the conclusion that a site for the coupling of photosynthetic phosphorylation with electron transport must exist between the 2 photochemical systems.
在光合作用受损的莱茵衣藻突变株中,研究了与P700相关的电子传递活性和吸光度变化。该突变株ac-8oa不能利用来自水、染料和抗坏血酸的电子还原NADP,但具有相当可观的希尔活性。该突变株没有显示出P700特有的吸光度变化。尽管ac-8oa不能进行循环光合磷酸化,但当提供铁氰化物作为电子受体时,它能够合成ATP。这些观察结果得出结论,光合磷酸化与电子传递的偶联位点必定存在于两个光化学系统之间。