Corporate Research Department and Monsanto Agricultural Products Company, Monsanto Company, St. Louis, Missouri 63166.
Plant Physiol. 1975 Jun;55(6):1048-53. doi: 10.1104/pp.55.6.1048.
Fourier transform (13)C nuclear magnetic resonance spectra have been obtained of intact, fresh soybean ovules (Glycine max L. cv. Dare) harvested from pods subtended by a trifoliolate exposed to (13)CO(2) 1 to 3 days earlier. The high resolution spectra are interpreted in terms of the labeled sugars and lipids in the ovule. Comparison of the spectra taken over the 3-day period permits qualitative estimates of sugar metabolism and rates of lipid synthesis. The spectra also contain information about the distribution of labels within the lipid chains. This information leads to a method of estimating the extent to which glucose degradation in the synthesizing soybean ovule is involved in the reactions of the phosphogluconate pathway.
傅里叶变换(13)C 核磁共振波谱已获得完整的,新鲜的大豆胚珠(Glycine max L. cv. Dare)收获豆荚下三叶暴露于(13)CO 2 1 至 3 天前。高分辨率谱解释的标签在胚珠的糖和脂质。在 3 天期间采取的光谱的比较允许定性估计糖代谢和脂质合成的速率。谱还包含有关标签在脂质链内的分布信息。该信息导致一种方法来估计在合成大豆胚珠中葡萄糖降解参与磷酸戊糖途径的反应的程度。