大肠杆菌中葡萄糖代谢的高分辨率碳-13核磁共振研究。
High-resolution 13C nuclear magnetic resonance studies of glucose metabolism in Escherichia coli.
作者信息
Ugurbil K, Brown T R, den Hollander J A, Glynn P, Shulman R G
出版信息
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3742-6. doi: 10.1073/pnas.75.8.3742.
Abstract
High-resolution 13C nuclear magnetic resonance spectra of suspensions of Escherichia coli cells have been obtained at 90.5 MHz by using the Fourier transform mode. Anaerobic cells incubated with [I-13C]glucose show a time course of glycolysis in which the alpha and beta glucose anomers disappear at different rates, lactate, succinate, acetate, alanine, and valine accumulate as end products of glycolysis, and fructose bisphosphate appears as an intermediate. It is shown that fructose bisphosphate is labeled at C-1 and C-6 during [I-13C]-glucose catabolism. Upon oxygenation, glutamate appears with the 13C ENRICHMENT AT THE C-4, C-3, and C-2 positions, with the C-4 most intense. From the position of the 13C label we conclude that valine is formed by condensation of pyruvate and that carbon enters the tricarboxylic acid cycle mainly through acetyl CoA.
摘要
利用傅里叶变换模式，在90.5兆赫兹下获得了大肠杆菌细胞悬浮液的高分辨率13C核磁共振谱。用[I-13C]葡萄糖培养的厌氧细胞显示出糖酵解的时间进程，其中α和β葡萄糖异头物以不同速率消失，乳酸、琥珀酸、乙酸、丙氨酸和缬氨酸作为糖酵解的终产物积累，果糖二磷酸作为中间产物出现。结果表明，在[I-13C]葡萄糖分解代谢过程中，果糖二磷酸在C-1和C-6处被标记。在充氧时，谷氨酸在C-4、C-3和C-2位置出现13C富集，其中C-4处的富集最为强烈。根据13C标记的位置，我们得出结论，缬氨酸是由丙酮酸缩合形成的，并且碳主要通过乙酰辅酶A进入三羧酸循环。
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