Department of Horticulture, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1975 Jul;56(1):83-7. doi: 10.1104/pp.56.1.83.
A particulate fraction from pollen tubes and ungerminated pollen of Lilium longiflorum incorporated (14)C-glucose from UDP-glucose-(14)C into a lipid fraction and into beta-1, 3-glucan. Partial hydrolysis of the glucan yielded laminaribiose as the only radioactive disaccharide. The preferred substrate was UDP-glucose, and enzyme activity was stimulated by glucose and by beta-linked di- and trisaccharides. Enzyme from growing pollen tubes synthesized beta-1, 3-glucan more rapidly and produced a higher proportion of alkali-insoluble glucan than did enzyme from ungerminated pollen. The onset of pollen tube growth may be dependent on altered activity of beta-1, 3-glucan synthase.
百合花粉管和未萌发花粉的颗粒部分将(14)C-葡萄糖掺入 UDP-葡萄糖-(14)C 中,形成脂类部分和β-1,3-葡聚糖。葡聚糖的部分水解仅产生作为唯一放射性二糖的纤维二糖。最优选的底物是 UDP-葡萄糖,并且酶活性被葡萄糖和β-连接的二糖和三糖所刺激。来自生长花粉管的酶比来自未萌发花粉的酶更快地合成β-1,3-葡聚糖,并且产生更高比例的碱不溶性葡聚糖。花粉管生长的开始可能依赖于β-1,3-葡聚糖合酶活性的改变。