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钙对燕麦胚芽鞘中吲哚乙酸诱导酸化的需求。

Calcium Requirement for Indoleacetic Acid-induced Acidification by Avena Coleoptiles.

机构信息

Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824.

出版信息

Plant Physiol. 1976 Mar;57(3):347-50. doi: 10.1104/pp.57.3.347.

Abstract

The ionic specificity of IAA-induced acidification by Avena coleoptiles was studied, using zwitterionic, presumably impermeant buffers. The acidification was almost totally dependent on divalent cations with an order of effectiveness of Ca(2+) >/= Sr(2+) > Mn(2+), Mg(2+); whereas other polyvalent cations tested were ineffective. The Ca(2+) response was IAA-dependent. The CaCl(2) concentration was optimal at 0.3 to 1 mm and inhibitory at higher concentrations. Sr(2+) inhibited Ca(2+)-dependent acidification and monovalent cations such as K(+) did not induce additional acidification in the presence of optimal CaCl(2). These data are consistent with a mechanism for IAA-induced acidification involving a Ca(2+) -H(+) exchange.

摘要

采用两性离子、可能不可渗透的缓冲液研究了 IAA 诱导的燕麦 coleoptiles 酸化的离子特异性。酸化几乎完全依赖于二价阳离子,其有效性顺序为 Ca(2+)>=Sr(2+)>Mn(2+)>Mg(2+);而其他测试的多价阳离子则无效。Ca(2+)反应是 IAA 依赖性的。CaCl(2)浓度在 0.3 至 1 mM 时最佳,在较高浓度时具有抑制作用。Sr(2+)抑制 Ca(2+)-依赖性酸化,并且在存在最佳 CaCl(2)的情况下,单价阳离子如 K(+)不会诱导额外的酸化。这些数据与涉及 Ca(2+) -H(+)交换的 IAA 诱导酸化的机制一致。

相似文献

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Bound indoleacetic Acid in Avena coleoptiles.燕麦胚芽鞘中的结合态吲哚乙酸
Plant Physiol. 1966 Feb;41(2):335-42. doi: 10.1104/pp.41.2.335.

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Hydrogen ion buffers for biological research.用于生物学研究的氢离子缓冲剂。
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