绿豆检测法检测马隆菌素诱导的生长刺激作用
A Mung Bean Assay for Malformin-induced Growth Stimulation.
机构信息
Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907.
出版信息
Plant Physiol. 1976 Mar;57(3):365-8. doi: 10.1104/pp.57.3.365.
A bioassay employing green or etiolated cuttings of Phaseolus aureus Roxb. was developed for determining malformin-induced growth stimulation in light. Growth enhancement of green cuttings was more rapid and relatively greater than that of etiolated cuttings. Cuttings from green seedlings responded less as seedlings aged; those from etiolated seedlings responded more. Malformin also stimulated the growth of green or etiolated seedlings in light. Most growth enhancement induced by malformin occurred in the upper 1 cm of the stem. Using green cuttings, malformin stimulated stem elongation relatively more when cotyledons, leaves, or especially apical buds were removed. Although malformin failed to stimulate elongation of 2-cm stem sections "floated" on solutions in Petri dishes, it stimulated elongation of sections when they were upright. High concentrations of indoleacetic acid inhibited growth enhancement by malformin. When gibberellin and malformin were combined, growth enhancement was nearly additive.
采用金雀花绿苗或黄化苗切段进行的生物测定表明，黄曲霉素诱导光下生长刺激。绿苗切段的生长增强比黄化苗切段更为迅速和相对较大。随着幼苗的老化，来自绿苗的切段反应较少；而来自黄化苗的切段反应较多。黄曲霉素也刺激绿苗或黄化苗在光下生长。黄曲霉素诱导的大多数生长增强发生在茎的上部 1 厘米处。使用绿苗切段，当去除子叶、叶片或特别是顶芽时，黄曲霉素相对更刺激茎伸长。虽然黄曲霉素不能刺激漂浮在 Petri 盘溶液中的 2 厘米茎段的伸长，但当它们直立时，黄曲霉素刺激伸长。吲哚乙酸的高浓度抑制黄曲霉素诱导的生长增强。当赤霉素和黄曲霉素结合使用时，生长增强几乎是相加的。
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