Møller B L
Department of Chemistry, Royal Veterinary and Agricultural University, DK 1871 Copenhagen V, Denmark.
Plant Physiol. 1976 May;57(5):687-92. doi: 10.1104/pp.57.5.687.
Lysine catabolism in seedlings of barley (Hordeum vulgare L. var. Emir) was studied by direct injection of the following tracers into the endosperm of the seedlings: aspartic acid-3-(14)C, 2-aminoadipic acid-1-(14)C, saccharopine-(14)C, 2,6-diaminopimelic acid-1-(7)-(14)C, and lysine-1-(14)C. Labeled saccharopine was formed only after the administration of either labeled 2,6-diaminopimelic acid or labeled lysine to the seedlings. The metabolic fate of the other tracers administered also supported a catabolic lysine pathway via saccharopine, and apparently proceeding by a reversal of some of the biosynthetic steps of the 2-aminoadipic acid pathway known from lysine biosynthesis in most fungi. Pipecolic acid seems not to be on the main pathway of l-lysine catabolism in barley seedlings.
通过向大麦(Hordeum vulgare L. var. Emir)幼苗胚乳中直接注射以下示踪剂,研究了其赖氨酸分解代谢:天冬氨酸-3-(14)C、2-氨基己二酸-1-(14)C、酵母氨酸-(14)C、2,6-二氨基庚二酸-1-(7)-(14)C和赖氨酸-1-(14)C。仅在向幼苗施用标记的2,6-二氨基庚二酸或标记的赖氨酸后,才形成标记的酵母氨酸。所施用的其他示踪剂的代谢命运也支持通过酵母氨酸的赖氨酸分解代谢途径,并且显然是通过逆转大多数真菌赖氨酸生物合成中已知的2-氨基己二酸途径的一些生物合成步骤进行的。哌啶酸似乎不在大麦幼苗L-赖氨酸分解代谢的主要途径上。