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本文引用的文献

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The composition of soluble nucleotides in the developing wheat grain.发育小麦籽粒中可溶性核苷酸的组成。
Plant Physiol. 1968 Jan;43(1):41-9. doi: 10.1104/pp.43.1.41.
2
The apyrase activity of potato extract.马铃薯提取物的腺苷三磷酸双磷酸酶活性。
Arch Biochem Biophys. 1962 Jun;97:609-10. doi: 10.1016/0003-9861(62)90131-5.
3
KINETIC STUDIES AND PROPERTIES OF POTATO APYRASE.马铃薯焦磷酸酶的动力学研究及性质
Arch Biochem Biophys. 1965 Jan;109:173-84. doi: 10.1016/0003-9861(65)90303-6.
4
THE PROBLEM OF HALTING ENZYME ACTION WHEN EXTRACTING PLANT TISSUES.提取植物组织时酶活性停止的问题。
Anal Biochem. 1964 Dec;9:431-42. doi: 10.1016/0003-2697(64)90204-0.
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RESOLUTION OF COMPLEX NUCLEOTIDE MIXTURES BY TWO-DIMENSIONAL ANION-EXCHANGE THIN-LAYER CHROMATOGRAPHY.通过二维阴离子交换薄层色谱法解析复杂核苷酸混合物
J Chromatogr. 1964 Oct;16:126-9. doi: 10.1016/s0021-9673(01)82446-8.
6
ION-EXCHANGE CHROMATOGRAPHY OF NUCLEOTIDES ON POLY-(ETHYLENEIMINE)-CELLULOSE THIN LAYERS.核苷酸在聚（乙烯亚胺）-纤维素薄层上的离子交换色谱法
J Chromatogr. 1964 Oct;16:111-25. doi: 10.1016/s0021-9673(01)82445-6.
7
The acid-soluble nucleotides of mature pea seeds.成熟豌豆种子的酸溶性核苷酸。
Biochem J. 1962 Dec;85(3):633-40. doi: 10.1042/bj0850633.
8
Splitting of the terminal phosphate group of adenosine triphosphate by potato apyrase.马铃薯焦磷酸酶催化三磷酸腺苷末端磷酸基团的裂解
Biochim Biophys Acta. 1962 Feb 12;57:158-60. doi: 10.1016/0006-3002(62)91096-x.
9
Fluorometric measurement of reduced pyridine nucleotide in cellular and subcellular particles.细胞及亚细胞颗粒中还原型吡啶核苷酸的荧光测定
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10
A fluorimetric method for the quantitative microanalysis of adenine and pyridine nucleotides.一种用于腺嘌呤和吡啶核苷酸定量微量分析的荧光法。
Anal Biochem. 1962 May;3:369-82. doi: 10.1016/0003-2697(62)90065-9.

可能的酸稳定酶在高等植物组织中核苷二磷酸和三磷酸提取过程中的干扰。

Possible Interference by an Acid-stable Enzyme during the Extraction of Nucleoside Di- and Triphosphates from Higher Plant Tissues.

机构信息

Department of Cellular and Molecular Biology, Division of Biological Sciences, University of Michigan, Ann Arbor, Michigan 48109.

出版信息

Plant Physiol. 1976 Sep;58(3):320-3. doi: 10.1104/pp.58.3.320.

DOI:10.1104/pp.58.3.320
PMID:16659671
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC542239/
Abstract

Acid extracts from tissues of two solanaceous plants were found to contain a heat-labile, nondialyzable factor which hydrolyzes nucleoside di- and triphosphates to nucleoside monophosphates. This acid-resistant factor shows optimal ATP-hydrolyzing activity at pH 5, whereas practically no activity was detected below pH 3 and above pH 9. It does not hydrolyze sugar phosphates, nucleoside monophosphates, uridine diphosphoglucose, and phosphoenolpyruvate. In order to estimate quantitatively the amount of nucleoside di- and triphosphates in a plant extract, care must be taken to circumvent possible interference by this factor. This is achieved by carefully maintaining the extract below pH 3.

摘要

从两种茄科植物的组织中提取的酸液中发现有一种热不稳定、不可透析的因子，它能将核苷二磷酸和三磷酸水解成核苷一磷酸。这种酸抗性因子在 pH5 时表现出最佳的 ATP 水解活性，而在 pH3 以下和 pH9 以上几乎检测不到活性。它不能水解糖磷酸、核苷一磷酸、尿苷二磷酸葡萄糖和磷酸烯醇丙酮酸。为了定量估计植物提取物中核苷二磷酸和三磷酸的含量，必须小心避免这种因子可能造成的干扰。这可以通过仔细将提取物的 pH 值保持在 3 以下来实现。