Department of Botany, University of Iowa, Iowa City, Iowa 52242.
Plant Physiol. 1977 Feb;59(2):207-10. doi: 10.1104/pp.59.2.207.
The choice of leaf-killing technique was found to affect significantly the distribution of label among early labeled photosynthetic products in two C(4) plants, Portulaca oleracea and Zea mays. The major effect of these procedures was on the amount of amino acids present, particularly alanine, and the ratio of malate to aspartate. Killing Portulaca leaves in alcohol generally results in more alanine and the predominance of malate over aspartate. When the leaves are killed by immediate freezing, however, aspartate contained more radioactivity than malate, and alanine was present in much reduced amounts. The various methods also differ in the relative amounts of C(3) cycle compounds and other, secondary intermediates which were obtained.
叶杀技术的选择被发现会显著影响两种 C4 植物(马齿苋和玉米)中早期标记光合作用产物的标签分布。这些程序的主要影响是对存在的氨基酸数量,特别是丙氨酸,以及苹果酸与天冬氨酸的比例。通常,用酒精杀死马齿苋叶会导致更多的丙氨酸和苹果酸的优势超过天冬氨酸。然而,当叶片立即冷冻杀死时,天冬氨酸比苹果酸含有更多的放射性,并且丙氨酸的含量大大减少。各种方法在获得的 C3 循环化合物和其他次要中间产物的相对量上也有所不同。