测定葡萄叶片中光合丝氨酸和磷酸甘油酸中的 C 分布。
Determination of C distribution in photosynthetic serine and phosphoglycerate from grape leaves.
机构信息
Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland.
出版信息
Plant Physiol. 1983 Nov;73(3):579-81. doi: 10.1104/pp.73.3.579.
Serine and phosphoglyceric acid are the classical marker intermediates of photorespiration and reductive carbon assimilation in C(3) plants. The present paper introduces a new and fast method for the determination of (14)C distribution in these compounds by selective elimination of C-3 (NaIO(4)) or C-1 (ninhydrin/ceric sulfate). Reproducibility of the procedure was found to be better than +/-1% upon degradation of [U-(14)C]serine and [U-(14)C]glycerate standards.Determination of labeling patterns in (dephosphorylated) phosphoglycerate and serine, extracted from grape leaf discs (Vitis vinifera L.) after administration of (14)CO(2) for 1 minute showed that (14)C distribution in serine changes considerably with changing assimilatory conditions. Interpretations of data basing on unusual labeling patterns of serine must therefore be accepted with caution.
丝氨酸和磷酸甘油酸是 C3 植物光呼吸和还原碳同化的经典标记中间产物。本文介绍了一种新的快速方法，通过选择性消除 C-3（高碘酸钠）或 C-1（水合茚三酮/硫酸铈）来测定这些化合物中（14）C 的分布。发现该程序在降解 [U-（14）C]丝氨酸和 [U-（14）C]甘油酸标准品时的重现性优于 +/-1%。用（14）CO2 处理 1 分钟后，从葡萄叶片（Vitis vinifera L.）中提取（去磷酸化）磷酸甘油酸和丝氨酸，测定其标记模式，结果表明，丝氨酸中（14）C 的分布随同化条件的变化而发生很大变化。因此，基于丝氨酸异常标记模式的数据分析必须谨慎接受。
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