Chen M, Loewus F A
Department of Agricultural Chemistry, Washington State University, Pullman, Washington 99164.
Plant Physiol. 1977 Apr;59(4):653-7. doi: 10.1104/pp.59.4.653.
Germinating Lilium longiflorum pollen absorbs and metabolizes myo-inositol-2-(3)H (MI-2-(3)H) with a pronounced lag when label is supplied from the beginning of germination. If MI-2-(3)H is given after 3 hours of germination, incorporation of labeled metabolic products into pollen tube polysaccharides is constant over a range of 0.56 mm to 2.78 mm MI. When MI-2-(3)H is supplied as a 0.5-hour pulse 3 hours after germination, the proper precursor-product relationship to tube wall polysaccharides is observed. Replacing 10% of the germination media with sigmatic exudate from a compatible cultivar hastens germination and tube elongation. Enhanced MI metabolism accompanies tube growth in this exudate-enriched media.
从萌发开始供应标记物时,萌发的麝香百合花粉吸收并代谢肌醇-2-(³H)(MI-2-(³H)),存在明显的延迟。如果在萌发3小时后给予MI-2-(³H),标记代谢产物掺入花粉管多糖的过程在0.56毫米至2.78毫米MI范围内是恒定的。当在萌发3小时后以0.5小时脉冲形式供应MI-2-(³H)时,观察到与管壁多糖的正确前体-产物关系。用来自相容品种的西格玛渗出液替代10%的萌发培养基可加速萌发和花粉管伸长。在这种富含渗出液的培养基中,MI代谢增强伴随着花粉管生长。