Faculty of Pharmaceutical Sciences, University of Toyama, Gofuku, 930, Toyama, Japan.
Planta. 1979 Jan;146(1):49-54. doi: 10.1007/BF00381254.
Biosynthesis of the cell wall in carrot cells (Daucus carota L.) cultured in a synthetic liquid medium was studied by measuring the incorporation of radioactive glucose and myo-inositol (MI). When the cells were fed with [(14)C]glucose in the presence of 0.01% MI, the label soon appeared in the neutral sugars in the cell wall but little radioactivity was found in the uronic-acid residues even after a prolonged incubation. On the other hand, radioactivity derived from [(3)H]MI was found to be distributed among uronic acids and pentoses but not in the hexose residues in the wall. The data indicate that MI is an important intermediate for the synthesis of acidic sugars in the wall of cultured carrot cells.
用放射性标记的葡萄糖和肌醇（MI）研究了在合成液体培养基中培养的胡萝卜细胞（Daucus carota L.）的细胞壁生物合成。当细胞在 0.01% MI 的存在下用 [(14)C]葡萄糖喂养时，标记很快出现在细胞壁的中性糖中，但即使在长时间孵育后，也几乎没有发现[14C]在糖醛酸残基中。另一方面，发现 [(3)H]MI 的放射性分布在糖醛酸和戊糖中，但不在细胞壁中的己糖残基中。这些数据表明 MI 是培养的胡萝卜细胞细胞壁中酸性糖合成的重要中间产物。
