尿苷 5′-焦磷酸葡萄糖醛酸吡喃糖基转移酶的分离及其用焦磷酸检测。
Isolation of uridine 5'-pyrophosphate glucuronic Acid pyrophosphorylase and its assay using p-pyrophosphate.
机构信息
Department of Horticulture, University of Illinois, Urbana, Illinois 61801.
出版信息
Plant Physiol. 1977 Jun;59(6):1082-4. doi: 10.1104/pp.59.6.1082.
Abstract
A procedure was devised to detect and assay uridine 5'-pyrophosphate (UDP)-glucuronic acid pyrophosphorylase in plant extracts. Substrates are UDP-glucuronic acid and (32)P-pyrophosphate, and the (32)P-uridine 5'-triphosphate produced is selectively adsorbed to charcoal. The charcoal adsorption procedure is a modification of that used to determine (32)P-adenosine 5'-triphosphate produced by adenosine 5'-pyrophosphate glucose pyrophosphorylase, and the modification greatly improves the retention of uridine 5'-triphosphate.
摘要
设计了一种方法来检测和测定植物提取物中的尿苷 5'-焦磷酸（UDP）-葡萄糖醛酸焦磷酸化酶。底物为 UDP-葡萄糖醛酸和（32）P-焦磷酸，生成的（32）P-尿苷 5'-三磷酸被选择性地吸附到活性炭上。该活性炭吸附程序是对用于测定由腺苷 5'-焦磷酸葡萄糖焦磷酸化酶产生的（32）P-腺苷 5'-三磷酸的程序的修改，该修改大大提高了尿苷 5'-三磷酸的保留率。
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本文引用的文献
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Enzymic synthesis of uridine diphosphate glucuronic acid and uridine diphosphate galacturonic acid with extracts from Phaseolus aureus seedlings.用金甲豆幼苗提取物进行尿苷二磷酸葡糖醛酸和尿苷二磷酸半乳糖醛酸的酶促合成。
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Biochem J. 1972 Jun;128(2):215-27. doi: 10.1042/bj1280215.
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