Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706.
Plant Physiol. 1977 Oct;60(4):606-8. doi: 10.1104/pp.60.4.606.
A simple, rapid procedure is described for evaluating ozone injury to leaves of Phaseolus vulgaris L. cv. Pinto. Leaf chlorophyll is extracted with ethanol and analyzed spectrophotometrically; the concentration is expressed on the basis of leaf dry weight.The per cent chlorophyll reduction of ozone-injured leaves was highly correlated with the per cent visible necrosis and chlorosis (r = 0.96). The variability in injury estimates with chlorophyll analysis was slightly less than with visual evaluation. An evaluation of chlorophyll a and b concentrations separately showed that the chlorophyll a/b ratio decreased with increasing amounts of injury. Chlorophyll determinations made for leaves harvested at intervals after an ozone treatment indicated that maximum chlorophyll reduction had occurred by 4 days.This procedure for measuring ozone injury should be useful in eliminating the human bias associated with visible estimates of injury.
描述了一种简单、快速的方法来评估臭氧对菜豆（Phaseolus vulgaris L. cv. Pinto）叶片的伤害。用乙醇提取叶片叶绿素，然后分光光度法分析；浓度以叶片干重为基础表示。臭氧损伤叶片的叶绿素减少百分比与可见坏死和黄化的百分比高度相关（r = 0.96）。用叶绿素分析评估损伤的变异性略小于用视觉评估。单独评估叶绿素 a 和 b 的浓度表明，随着损伤程度的增加，叶绿素 a/b 比值降低。臭氧处理后每隔一段时间收获的叶片进行叶绿素测定表明，叶绿素最大减少发生在 4 天。这种测量臭氧损伤的方法应该有助于消除与可见损伤估计相关的人为偏见。
