叶绿素荧光测定法检测完整植物的臭氧伤害。
Chlorophyll fluorescence assay for ozone injury in intact plants.
机构信息
Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305.
出版信息
Plant Physiol. 1978 Jan;61(1):80-4. doi: 10.1104/pp.61.1.80.
Abstract
A chlorophyll fluorescence induction (Kautsky effect) assay predicted ozone-induced injury in bean leaves (Phaseolus vulgaris) at least 20 hours before any visible sign of leaf necrosis. The extent of injury, which could be predicted during exposure to ozone, depended on concentration, exposure time, and leaf development stage. Much more injury occurred in light than in darkness and long exposures to lower ozone concentrations were more injurious than brief exposures to higher ones. The first detectable effect was on the photosynthetic water-splitting enzyme systems, followed by inhibition of electron transport between the photosystems. The fluorescence assay provides a simple, rapid, nondestructive method for observing effects of ozone on plants.
摘要
叶绿素荧光诱导（卡尔斯基效应）分析可在肉眼观察到叶片坏死前至少 20 小时预测臭氧对菜豆叶片（Phaseolus vulgaris）的损伤。在臭氧暴露期间可预测的损伤程度取决于浓度、暴露时间和叶片发育阶段。在光下比在黑暗中发生的损伤更大，并且较长时间暴露于较低浓度的臭氧比短暂暴露于较高浓度的臭氧更有害。首先检测到的是对光合作用水分解酶系统的影响，随后抑制了光系统之间的电子传递。荧光分析为观察臭氧对植物的影响提供了一种简单、快速、非破坏性的方法。
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Changes in respiration, photosynthesis, adenosine 5'-triphosphate, and total adenylate content of ozonated pinto bean foliage as they relate to symptom expression.臭氧处理的菜豆叶片的呼吸作用、光合作用、腺苷三磷酸及总腺苷酸含量的变化与症状表现的关系。
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