Hillson T D, Lamotte C E
Department of Botany and Plant Pathology, Iowa State University, Ames, Iowa 50011.
Plant Physiol. 1977 Dec;60(6):881-4. doi: 10.1104/pp.60.6.881.
Stem segments were excised from plants of Wisconsin 38 tobacco (Nicotiana tabacum L.) in three regions differing in their distance below the inflorescence. They were cultured in vitro in 8- or 16-hr days. After 8 weeks, floral and vegetative buds were counted, and extent of floral development was assessed. Kinetin at 10(-5)m inhibited formation and development of floral buds regardless of indoleacetic acid concentration. Supplied at this concentration with adequate auxin, kinetin stimulated vegetative bud formation and may have caused floral bud abortion. Indoleacetic acid (>/= 10(-6)m) inhibited vegetative and floral bud formation when supplied with low kinetin concentration (</= 10(-7)m) but did not affect floral development. When supplied with high kinetin concentration (>/= 10(-6)m), it inhibited floral bud formation and stimulated vegetative bud formation. More floral buds were formed in 16-hr days than in 8-hr days. Few formed on explants other than those derived from the region nearest the inflorescence regardless of other treatment.
从威斯康星38烟草（Nicotiana tabacum L.）植株上切取茎段，取材于花序以下不同距离的三个部位。将其在16小时光照或8小时光照条件下进行离体培养。8周后，统计花芽和叶芽数量，并评估花芽发育程度。无论吲哚乙酸浓度如何，10⁻⁵m的激动素均抑制花芽的形成和发育。在此浓度下，若同时供应适量生长素，激动素会刺激叶芽形成，并可能导致花芽败育。当供应低浓度激动素（≤10⁻⁷m）时，吲哚乙酸（≥10⁻⁶m）抑制叶芽和花芽形成，但不影响花芽发育。当供应高浓度激动素（≥10⁻⁶m）时，吲哚乙酸抑制花芽形成并刺激叶芽形成。16小时光照条件下形成的花芽比8小时光照条件下更多。无论其他处理如何，除了取自最靠近花序部位的外植体，其他外植体上形成的花芽很少。
