McDaniel C N, Sangrey K A, Jegla D E
Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180-3590.
Dev Biol. 1989 Aug;134(2):473-8. doi: 10.1016/0012-1606(89)90120-6.
The developmental fates of shoots regenerated in culture and in situ by stem tissues of Nicotiana tabacum cv. Wisconsin 38 from different positions along the main axes of plants at different ages have been characterized. It was expected that explants from vegetative plants would not have the capacity to produce floral shoots. Contrary to the expected result, a small percentage (about 0.2%) of the shoots formed from cultured stem explants taken from young, vegetative plants were floral, i.e., produced a small number of nodes and then a flower. A larger percentage (about 2%) of the shoots formed by explants from the same region of plants which had flowered were floral. The largest percentage (76%) of floral shoots arose from explants taken from the inflorescence. Internode cells which were stimulated to divide and undergo organogenesis in situ after decapitation of the plant also produced few-noded, floral shoots with apical internode tissues producing many such floral shoots and basal internode tissues producing few such floral shoots. These results indicate that the capacity to form a flower is a visible expression of a cryptic developmental state which is quantitatively but not qualitatively controlled in time and space.
已对烟草品种威斯康星38不同年龄植株主轴不同位置的茎组织在离体培养和原位条件下再生芽的发育命运进行了表征。预计来自营养生长植株的外植体没有产生花芽的能力。与预期结果相反，从幼龄营养生长植株上获取的培养茎外植体形成的芽中有一小部分（约0.2%）是花芽，即产生少量节然后形成一朵花。来自已开花植株相同区域的外植体形成的芽中较大比例（约2%）是花芽。最大比例（76%）的花芽来自取自花序的外植体。在植株去顶后被刺激在原位分裂并进行器官发生的节间细胞也产生了少节的花芽，顶端节间组织产生许多这样的花芽，而基部节间组织产生的此类花芽较少。这些结果表明，形成花的能力是一种隐性发育状态的可见表现，这种状态在时间和空间上受到定量而非定性的控制。
