在芥菜子叶苯丙氨酸解氨酶合成的调节中不存在光敏色素破坏现象。
Absence of pfr destruction in the modulation of phenylalanine ammonia-lyase synthesis of mustard cotyledons.
作者信息
Tong W F, Schopfer P
机构信息
Biologisches Institut II, Universität Freiburg im Breisgau, Germany.
出版信息
Plant Physiol. 1978 Jan;61(1):59-61. doi: 10.1104/pp.61.1.59.
The relationship between the amount of active phytochrome (Pfr) produced by 5-minute light pulses and the rate of subsequent enzyme accumulation (phenylalanine ammonia-lyase, EC 4.3.1.5) of mustard (Sinapis alba L.) cotyledons was investigated. The response rapidly adjusts to changes of the Pfr level produced by light pulses of different wavelengths. Regardless of total phytochrome levels in the cotyledons, response adjustments to new photostationary states (phi(lambda)) are correlated with phi(alpha) values. On the other hand, the kinetics of enzyme accumulation shows no influence of Pfr destruction as determined spectrophotometrically (tau((1/2)) = 45 min) in the same organ (see Schäfer et al. 1973 Photochem Photobiol 18: 331-334). It is concluded that the phytochrome molecules involved in the regulation of this response by light pulses comprise a small fraction of the total phytochrome of the cotyledons. In contrast to bulk phytochrome this fraction appears to be not subject to Pfr destruction.
研究了5分钟光脉冲产生的活性光敏色素（Pfr）量与芥菜（Sinapis alba L.）子叶随后的酶积累速率（苯丙氨酸解氨酶，EC 4.3.1.5）之间的关系。该反应能迅速适应不同波长光脉冲产生的Pfr水平变化。无论子叶中光敏色素的总量如何，对新的光稳态（phi(lambda)）的反应调节都与phi(alpha)值相关。另一方面，酶积累的动力学不受分光光度法测定的Pfr破坏的影响（tau((1/2)) = 45分钟），在同一器官中也是如此（见Schäfer等人，1973年，《光化学与光生物学》18: 331 - 334）。得出的结论是，参与光脉冲调节此反应的光敏色素分子仅占子叶总光敏色素的一小部分。与大量光敏色素不同，这一部分似乎不受Pfr破坏的影响。
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