Mok M C, Mok D W, Armstrong D J
Department of Horticulture and Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331.
Plant Physiol. 1978 Jan;61(1):72-5. doi: 10.1104/pp.61.1.72.
The activities of eight cytokinins in promoting callus growth were tested in two Phaseolus genotypes, P. vulgaris L. var. Great Northern, and P. lunatus L. var. Kingston. The structural feature which contributes to the major genotypic difference in cytokinin structure-activity relationships is the presence or absence of a double bond at the 2,3-position of the isoprenoid N(6) side chain. In Kingston, trans-zeatin was 3-fold more active than dihydrozeatin and 30-fold more active than cis-zeatin. The activities of N(6)-(Delta(2)-isopentenyl)adenine and N(6)-isopentyladenine were nearly the same. In Great Northern, however, dihydrozeatin was at least 30-fold more active than both trans-zeatin and cis-zeatin, and N(6)-isopentyladenine was 100-fold more active than N(6)-(Delta(2)-isopentenyl)adenine. The results suggest the possibility of employing cytokinin structure-activity relationships in distinguishing genotypic differences in cytokinin function and metabolism.
在两种菜豆基因型（普通菜豆L.变种大北方和利马豆L.变种金斯敦）中测试了8种细胞分裂素促进愈伤组织生长的活性。细胞分裂素结构-活性关系中导致主要基因型差异的结构特征是类异戊二烯N(6)侧链2,3位双键的有无。在金斯敦，反式玉米素的活性比二氢玉米素高3倍，比顺式玉米素高30倍。N(6)-(Δ2-异戊烯基)腺嘌呤和N(6)-异戊基腺嘌呤的活性几乎相同。然而，在大北方，二氢玉米素的活性比反式玉米素和顺式玉米素至少高30倍，N(6)-异戊基腺嘌呤的活性比N(6)-(Δ2-异戊烯基)腺嘌呤高100倍。结果表明，利用细胞分裂素结构-活性关系来区分细胞分裂素功能和代谢的基因型差异是可能的。
