Aung L H
Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061.
Plant Physiol. 1978 Aug;62(2):276-9. doi: 10.1104/pp.62.2.276.
Hormonal and plant factors regulating the development of the inhibited cotyledonary buds of Lycopersicon esculentum Mill. cv. ;Fireball' seedlings were studied. Excision of the immature plumular leaves of 5- to 20- millimeter length significantly stimulated bud development after 2 to 4 days, but excision of leaves exceeding 20-millimeter length was without effect. Apical application of 20 microliters of 5 millimolar abscisic acid significantly promoted development of the cotyledonary buds after 6 days. A subapical ring of 0.1 millimolar concentration of 2,3,5-triiodobenzoic acid (TIBA) in lanolin significantly promoted cotyledonary bud development after 11 days. Twenty microliters of 0.1 millimolar 6-benzylaminopurine (BAP) applied directly to the cotyledonary bud loci significantly promoted bud development, but 1 micromolar gibberellin A(4/7) was ineffective. Application of 0.1 millimolar BAP in lanolin to the petiole or hypocotyl was ineffective. However, application of 0.1 millimolar TIBA as a ring around the petioles of the cotyledons or 1-centimeter on the hypocotyl below the cotyledons significantly promoted cotyledonary bud development.
对调节番茄(Lycopersicon esculentum Mill. cv.‘火球’)幼苗受抑制子叶芽发育的激素和植物因子进行了研究。切除长度为5至20毫米的未成熟胚叶,在2至4天后能显著刺激芽的发育,但切除长度超过20毫米的叶子则无效果。在顶端施用20微升5毫摩尔的脱落酸,6天后能显著促进子叶芽的发育。在羊毛脂中施用浓度为0.1毫摩尔的2,3,5 - 三碘苯甲酸(TIBA)形成亚顶端环,11天后能显著促进子叶芽的发育。直接将20微升0.1毫摩尔的6 - 苄基腺嘌呤(BAP)施用于子叶芽位点能显著促进芽的发育,但1微摩尔的赤霉素A(4/7)无效。将0.1毫摩尔的BAP以羊毛脂形式施用于叶柄或下胚轴无效。然而,在子叶叶柄周围以环的形式施用0.1毫摩尔的TIBA或在子叶下方的下胚轴上施用1厘米能显著促进子叶芽的发育。