葡萄科克氏循环中的酶：II. 精氨琥珀酸合成酶和裂解酶。
Enzymes of Krebs-Henseleit Cycle in Vitis vinifera L: II. Arginosuccinate Synthetase and Lyase.
机构信息
Department of Viticulture and Enology, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1978 Sep;62(3):340-3. doi: 10.1104/pp.62.3.340.
Abstract
Arginosuccinate (ASA) synthetase and lyase activities were detected in extracts from Vitis vinifera L. cv. Chenin blanc mature leaves and seedlings. Optimum reaction conditions for ASA synthetase were 10 millimolar l-citrulline, 7.5 millimolar l-aspartate, 3 to 4 millimolar ATP, 12 millimolar Mg(2+) (pH 7.5 to 8.0), enzyme extract up to equivalent of about 200 milligrams of fresh tissue, and incubation temperature of 38 to 40 C. Optimum reaction conditions for ASA lyase were 4 millimolar ASA-K salt (pH 7.3 to 7.8), amount of extract up to equivalent of about 180 milligrams of fresh tissue, and incubation temperature of 38 to 40 C.
摘要
从白诗南（Chenin blanc）葡萄成熟叶片和幼苗的提取物中检测到精氨酸琥珀酸（ASA）合成酶和裂解酶活性。ASA 合成酶的最佳反应条件为 10 毫摩尔 L-瓜氨酸、7.5 毫摩尔 L-天冬氨酸、3 至 4 毫摩尔 ATP、12 毫摩尔 Mg2+（pH7.5 至 8.0）、酶提取物相当于约 200 毫克新鲜组织，以及 38 至 40°C 的孵育温度。ASA 裂解酶的最佳反应条件为 4 毫摩尔 ASA-K 盐（pH7.3 至 7.8）、提取物量相当于约 180 毫克新鲜组织，以及 38 至 40°C 的孵育温度。
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Enzymes of Krebs-Henseleit Cycle in Vitis vinifera L: III. In Vivo and In Vitro Studies of Arginase.三、葡萄科的克雷布斯-海斯莱因循环酶：III.精氨酸酶的体内和体外研究。
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本文引用的文献
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