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水分胁迫下棉花叶片的脂类和表面蜡质合成。

Lipid and Surface Wax Synthesis in Water-stressed Cotton Leaves.

机构信息

Department of Botany and Microbiology, Auburn University Agricultural Experiment Station, Auburn, Alabama 36830.

出版信息

Plant Physiol. 1978 Nov;62(5):675-7. doi: 10.1104/pp.62.5.675.

DOI:10.1104/pp.62.5.675
PMID:16660581
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1092196/
Abstract

The incorporation of [2-(14)C]malonate and [1-(14)C]acetate into internal lipid and surface wax by cotton leaves (Gossypium hirsutum L. ;Deltapine') having water potentials of -8 to -15 bars (controls) and -19 to -32 bars (water-stressed) was compared. Lipid from stressed leaves contained a mean of 57% more radioactivity than corresponding controls for five experiments. Acetyl coenzyme A carboxylase was not limiting to fatty acid synthesis in water-stressed cotton leaves at the water potential levels tested, whereas fatty acid synthetase was stimulated. In four of six experiments, wax from stressed leaves contained a mean of 38% less radioactivity than nonstressed leaves when incubated 24 hours after rehydration. Evidence is presented to show that after a suitable period of rehydration, previously stressed cotton leaves produce more wax than leaves prior to stressing.

摘要

将 [2-(14)C]丙二酸盐和 [1-(14)C]乙酸盐掺入水势为-8 至-15 巴(对照)和-19 至-32 巴(水分胁迫)的棉花叶片(陆地棉 L.;Deltapine')的内部脂质和表面蜡中进行了比较。在五项实验中,与对照相比,胁迫叶片中的脂质平均含有 57%更多的放射性。在测试的水势水平下,乙酰辅酶 A 羧化酶对水分胁迫棉花叶片中的脂肪酸合成没有限制,而脂肪酸合成酶受到刺激。在六个实验中的四个实验中,与非胁迫叶片相比,胁迫叶片的蜡在复水 24 小时后平均含有 38%更少的放射性。有证据表明,在适当的复水期后,先前受到胁迫的棉花叶片比胁迫前的叶片产生更多的蜡。

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本文引用的文献

1
Sap Pressure in Vascular Plants: Negative hydrostatic pressure can be measured in plants.植物中的液流压力:在植物中可以测量到负静水压力。
Science. 1965 Apr 16;148(3668):339-46. doi: 10.1126/science.148.3668.339.
2
The effect of water stress on mitochondria of root cells: a biochemical and cytochemical study.水分胁迫对根细胞线粒体的影响:一项生化与细胞化学研究
Plant Physiol. 1970 Feb;45(2):173-7. doi: 10.1104/pp.45.2.173.
3
Further evidence for an elongation-decarboxylation mechanism in the biosynthesis of paraffins in leaves.叶片中石蜡生物合成中伸长-脱羧机制的进一步证据。
Plant Physiol. 1968 Mar;43(3):375-83. doi: 10.1104/pp.43.3.375.
4
Acetyl-coenzyme-A carboxylase of Candida Lipolytica. 1. Purification and properties of the enzyme.解脂假丝酵母的乙酰辅酶A羧化酶。1. 该酶的纯化及性质
Eur J Biochem. 1976 Dec;71(1):295-300. doi: 10.1111/j.1432-1033.1976.tb11115.x.
5
Mechanisms and regulation of biosynthesis of saturated fatty acids.饱和脂肪酸生物合成的机制与调控
Physiol Rev. 1976 Apr;56(2):339-417. doi: 10.1152/physrev.1976.56.2.339.