Nee M, Chiu L, Eisinger W
Department of Biology, University of Santa Clara, Santa Clara, California 95053.
Plant Physiol. 1978 Dec;62(6):902-6. doi: 10.1104/pp.62.6.902.
Ethylene inhibits polar cell expansion of etiolated pea (Pisum sativum) internode tissue and results in a radial cell expansion, swelling. A special gas-tight growth chamber was developed to monitor continuously the induction of cell expansion inhibition of excised internode segments in the presence of ethylene. Following a complex induction period which lasts about 3 hours, a low, but steady-state growth rate is achieved. Ethylene removal experiments indicate that the gas induces an irreversible change in the cell expansion mode. If the tissue was transferred to an ethylene-free chamber after the steady-state rate had been achieved, this rate continued in the absence of the gas. If the gas was removed during an intermediate phase, that intermediate rate of growth continued after removal. In other experiments, segments were incubated with intact apical hooks and the induction period was much shorter than with isolated segments. Changes in imino acid metabolism have been correlated with the induction of swelling. Although total proline and hydroxyproline levels were not affected by ethylene treatment, incorporation of [(14)C]proline into a wall-associated protein was inhibited during the period when swelling occurred. These results suggest that ethylene is affecting proline pool sizes.
乙烯抑制黄化豌豆（Pisum sativum）节间组织的极性细胞扩展，并导致径向细胞扩展、肿胀。开发了一种特殊的气密生长室，以连续监测在乙烯存在下切除的节间段细胞扩展抑制的诱导情况。在持续约3小时的复杂诱导期后，实现了低但稳定的生长速率。乙烯去除实验表明，该气体诱导细胞扩展模式发生不可逆变化。如果在达到稳态速率后将组织转移到无乙烯的室中，在没有该气体的情况下该速率仍会继续。如果在中间阶段去除该气体，去除后该中间生长速率仍会继续。在其他实验中，节段与完整的顶端弯钩一起培养，诱导期比分离的节段短得多。亚氨基酸代谢的变化与肿胀的诱导有关。尽管乙烯处理不影响总脯氨酸和羟脯氨酸水平，但在肿胀发生期间，[(14)C]脯氨酸掺入与细胞壁相关蛋白的过程受到抑制。这些结果表明乙烯正在影响脯氨酸库的大小。
