Department of Botany, Ohio University, Athens, Ohio 45701.
Plant Physiol. 1978 Dec;62(6):949-53. doi: 10.1104/pp.62.6.949.
The transport of serine into tobacco cells (Nicotiana tabacum L.) cultured in liquid medium was examined. Transport was inhibited approximately 50% by 2,4-dichlorophenoxyacetic acid, indoleacetic acid, alpha-naphthalene acetic acid, and kinetin at a concentration of 10 micrograms per milliliter. Transport was not inhibited by 2,6-dichlorophenoxyacetic acid and inhibited less than 25% by p-chlorophenoxyacetic acid at this concentration. Removal of 2,4-dichlorophenoxyacetic acid from the transport medium resulted in an alleviation of inhibition. Gibberellic acid at concentrations from 2 to 20 micrograms per milliliter stimulated transport.It was previously shown that inhibition of transport by La(3+) was due to removal of Ca(2+) from surface sites and inhibition of Ca(2+) uptake by cells. None of the growth regulators tested had any significant effect on Ca(2+) binding and/or transport.A contributing factor to the low transport rates in the absence of Ca(2+) is the increased rate of serine efflux. None of the growth regulators tested had any significant effect on the rate of serine efflux.
研究了在液体培养基中培养的烟草细胞（Nicotiana tabacum L.）中丝氨酸的转运。在 10 微克/毫升的浓度下，2,4-二氯苯氧乙酸、吲哚乙酸、α-萘乙酸和激动素大约抑制了 50%的转运。2,6-二氯苯氧乙酸不抑制转运，在该浓度下抑制作用小于 25%。在运输培养基中去除 2,4-二氯苯氧乙酸可减轻抑制作用。赤霉素在 2 至 20 微克/毫升的浓度下刺激转运。之前的研究表明，La3+对转运的抑制作用是由于表面位点的 Ca2+被去除以及细胞对 Ca2+摄取的抑制。测试的生长调节剂均对 Ca2+结合和/或转运没有显著影响。在没有 Ca2+的情况下，转运速率较低的一个促成因素是丝氨酸外排速率的增加。测试的生长调节剂均对丝氨酸外排速率没有显著影响。
