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1
Intracellular location of carbonate dehydratase (carbonic anhydrase) in leaf tissue.碳酸酐酶在叶片组织中的细胞内定位。
Plant Physiol. 1979 Jan;63(1):216-7. doi: 10.1104/pp.63.1.216.
2
Carbonic anhydrase of spinach: studies on its location, inhibition, and physiological function.菠菜碳酸酐酶：关于其定位、抑制作用及生理功能的研究
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3
Isolation of intact chloroplasts and other cell organelles from spinach leaf protoplasts.从菠菜叶原生质体中分离完整的叶绿体和其他细胞器官。
Plant Physiol. 1976 Sep;58(3):309-14. doi: 10.1104/pp.58.3.309.
4
Subcellular distribution of carbonic anhydrase in Solanum tuberosum L. leaves: characterization of two compartment-specific isoforms.马铃薯叶片中碳酸酐酶的亚细胞分布：两种区室特异性同工型的特性分析
Planta. 1996;199(1):79-88. doi: 10.1007/BF00196884.
5
Regulation of chloroplastic carbonic anhydrase : effect of magnesium.叶绿体碳酸酐酶的调节：镁的影响。
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6
Multiplicity of soluble glucan-synthase activity in spinach leaves: Enzyme pattern and intracellular location.菠菜叶片中可溶性葡聚糖合成酶活性的多样性：酶谱和细胞内定位。
Planta. 1991 Sep;185(2):220-6. doi: 10.1007/BF00194064.
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8
Spinach carbonic anhydrase primary structure deduced from the sequence of a cDNA clone.从一个cDNA克隆序列推导的菠菜碳酸酐酶一级结构。
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9
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10
Purification of a carbonic anhydrase from the inner ear of the guinea pig.从豚鼠内耳中纯化一种碳酸酐酶。
Proc Natl Acad Sci U S A. 1977 Mar;74(3):892-6. doi: 10.1073/pnas.74.3.892.

引用本文的文献

1
The Flaveria bidentis beta-carbonic anhydrase gene family encodes cytosolic and chloroplastic isoforms demonstrating distinct organ-specific expression patterns.黄顶菊β-碳酸酐酶基因家族编码胞质和叶绿体同工型，表现出不同的器官特异性表达模式。
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2
Spinach chloroplastic carbonic anhydrase: nucleotide sequence analysis of cDNA.菠菜叶绿体碳酸酐酶：cDNA 的核苷酸序列分析。
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3
Carbonic Anhydrase-Dependent Inorganic Carbon Uptake by the Red Macroalga, Chondrus crispus.碳酸酐酶依赖的红藻角叉菜对无机碳的摄取。
Plant Physiol. 1987 Apr;83(4):735-8. doi: 10.1104/pp.83.4.735.
4
Molecular comparison of carbonic anhydrase from Flaveria species demonstrating different photosynthetic pathways.展示不同光合途径的黄顶菊属物种碳酸酐酶的分子比较
Plant Mol Biol. 1995 Oct;29(2):353-65. doi: 10.1007/BF00043658.
5
Arabidopsis thaliana carbonic anhydrase: cDNA sequence and effect of CO2 on mRNA levels.拟南芥碳酸酐酶：cDNA序列及二氧化碳对mRNA水平的影响。
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本文引用的文献

1
Isolation of intact chloroplasts and other cell organelles from spinach leaf protoplasts.从菠菜叶原生质体中分离完整的叶绿体和其他细胞器官。
Plant Physiol. 1976 Sep;58(3):309-14. doi: 10.1104/pp.58.3.309.
2
Carbonic anhydrase of spinach: studies on its location, inhibition, and physiological function.菠菜碳酸酐酶：关于其定位、抑制作用及生理功能的研究
Plant Physiol. 1975 Mar;55(3):468-74. doi: 10.1104/pp.55.3.468.
3
Plant Carbonic Anhydrases: I. Distribution of Types among Species.植物碳酸酐酶：I. 不同物种中的类型分布。
Plant Physiol. 1972 Aug;50(2):214-7. doi: 10.1104/pp.50.2.214.
4
CARBONIC ANHYDRASES FROM HUMAN ERYTHROCYTES. PREPARATION AND PROPERTIES OF TWO ENZYMES.人红细胞碳酸酐酶。两种酶的制备及性质
J Biol Chem. 1964 Apr;239:1065-78.
5
Evidence for the synthesis in vivo of proteins of the Calvin cycle and of the photosynthetic electron-transfer pathway on chloroplast ribosomes.叶绿体核糖体上卡尔文循环蛋白及光合电子传递途径蛋白在体内合成的证据。
Biochem Biophys Res Commun. 1967 Aug 23;28(4):604-10. doi: 10.1016/0006-291x(67)90356-7.
6
Carbonic anhydrase: a new method of detection on polyacrylamide gels using low-temperature fluorescence.碳酸酐酶：一种利用低温荧光在聚丙烯酰胺凝胶上进行检测的新方法。
Anal Biochem. 1971 Dec;44(2):388-91. doi: 10.1016/0003-2697(71)90224-7.
7
Accumulation of bicarbonate in intact chloroplasts following a pH gradient.在pH梯度作用下完整叶绿体中碳酸氢盐的积累。
Biochim Biophys Acta. 1972 Dec 14;283(3):430-41. doi: 10.1016/0005-2728(72)90260-5.
8
Intracellular distribution of carbonic anhydrase in spinach leaves.菠菜叶片中碳酸酐酶的细胞内分布
Biochim Biophys Acta. 1972 Feb 28;258(2):637-42. doi: 10.1016/0005-2744(72)90255-0.
9
[Purification and characterization of chloroplast carbonate dehydratase from leaves of Lactuca sativa (author's transl)].[莴苣叶片叶绿体碳酸酐酶的纯化与特性研究（作者译）]
Hoppe Seylers Z Physiol Chem. 1975 Nov;356(11):1733-41.

碳酸酐酶在叶片组织中的细胞内定位。

Intracellular location of carbonate dehydratase (carbonic anhydrase) in leaf tissue.

作者信息

Reed M L

机构信息

School of Biological Sciences, Macquarie University, North Ryde, New South Wales, Australia 2113.

出版信息

Plant Physiol. 1979 Jan;63(1):216-7. doi: 10.1104/pp.63.1.216.

DOI:10.1104/pp.63.1.216
PMID:16660683
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC542798/
Abstract

Two proteins which have carbonate dehydratase (carbonic anhydrase, EC 4.2.1.1) activity were shown to be in the chloroplasts and in the cytosol of leaves of Brassica chinensis, Spinacia oleracea, and in variegated leaves of Tradescantia albiflora and Hedera canariensis. The chloroplastic enzyme is smaller than the one in the cytosol, as it runs farther on gradient polyacrylamide gels. It was separated from the other by isolation of chloroplasts of Brassica and Spinacia on sucrose density gradients; approximately half of the total activity was in the chloroplasts.

摘要

在中国白菜、菠菜的叶片叶绿体和细胞质中，以及紫露草和加那利常春藤的杂色叶片中，发现了两种具有碳酸脱水酶（碳酸酐酶，EC 4.2.1.1）活性的蛋白质。叶绿体中的酶比细胞质中的酶小，因为它在梯度聚丙烯酰胺凝胶上迁移得更远。通过在蔗糖密度梯度上分离白菜和菠菜的叶绿体，将其与另一种酶分离；总活性的大约一半存在于叶绿体中。