碳酸酐酶在叶片组织中的细胞内定位。
Intracellular location of carbonate dehydratase (carbonic anhydrase) in leaf tissue.
作者信息
Reed M L
机构信息
School of Biological Sciences, Macquarie University, North Ryde, New South Wales, Australia 2113.
出版信息
Plant Physiol. 1979 Jan;63(1):216-7. doi: 10.1104/pp.63.1.216.
Abstract
Two proteins which have carbonate dehydratase (carbonic anhydrase, EC 4.2.1.1) activity were shown to be in the chloroplasts and in the cytosol of leaves of Brassica chinensis, Spinacia oleracea, and in variegated leaves of Tradescantia albiflora and Hedera canariensis. The chloroplastic enzyme is smaller than the one in the cytosol, as it runs farther on gradient polyacrylamide gels. It was separated from the other by isolation of chloroplasts of Brassica and Spinacia on sucrose density gradients; approximately half of the total activity was in the chloroplasts.
摘要
在中国白菜、菠菜的叶片叶绿体和细胞质中，以及紫露草和加那利常春藤的杂色叶片中，发现了两种具有碳酸脱水酶（碳酸酐酶，EC 4.2.1.1）活性的蛋白质。叶绿体中的酶比细胞质中的酶小，因为它在梯度聚丙烯酰胺凝胶上迁移得更远。通过在蔗糖密度梯度上分离白菜和菠菜的叶绿体，将其与另一种酶分离；总活性的大约一半存在于叶绿体中。
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