Cotton Physiology and Genetics Laboratory, Agricultural Research, Science and Education Administration, United States Department of Agriculture, Stoneville, Mississippi 38776.
Plant Physiol. 1979 Feb;63(2):260-3. doi: 10.1104/pp.63.2.260.
Patterns of accumulation and ontogenetic relationships among proteins of cotton (Gossypium hirsutum L.) seeds were examined between 10 days postanthesis and maturity (56 days). Total and extractable nitrogen contents were determined; alkali- and water-soluble proteins were assayed quantitatively and electrophoretically. Two alkali-soluble proteins present in the electrophoretogram of mature embryos first appeared at 21 days postanthesis; most of the final profile was established by 28 days. Except for minor changes centering around the 7th week of development, the pattern from 28 days to maturity was marked by intensification of bands. The quantity of water-soluble nitrogen increased through the first 21 days of development, then declined until 42 days, when it again began to increase; it reached its highest level at maturity. There was evidence of a high peptide content 7 weeks postanthesis.
研究了棉花（Gossypium hirsutum L.）种子在授粉后 10 天到成熟（56 天）期间的积累模式和个体发育关系。测定了总氮和可提取氮含量；定量和电泳分析了碱溶性和水溶性蛋白质。成熟胚电泳图谱中的两种碱溶性蛋白最初出现在授粉后 21 天；28 天时，大部分最终图谱已建立。除了在发育的第 7 周左右发生的微小变化外，从 28 天到成熟的图谱以带的增强为特征。水溶性氮的含量在发育的前 21 天增加，然后下降到 42 天，然后再次增加；在成熟时达到最高水平。授粉后 7 周有大量肽的证据。
