衰老前叶子中卟胆原脱氨酶活性的消失。
Disappearance of porphobilinogen deaminase activity in leaves before the onset of senescence.
机构信息
Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, Buenos Aires, Argentina.
出版信息
Plant Physiol. 1979 Jun;63(6):1154-7. doi: 10.1104/pp.63.6.1154.
Abstract
The activity of porphobilinogen deaminase was measured in young and senescent or mature leaves of pepper (Capsicum annuum), and poinsettia (Euphorbia pulcherrima). Whereas high activity was found in the crude extracts of the young leaves, almost no activity was found in the extracts of senescent or mature leaves. The decrease in deaminase activity was not due to the presence of an isolatable inhibitor. By purifying the crude enzyme extracts from leaves of different ages on DEAE-cellulose columns it was shown that the decrease in deaminase activity was due to a real decrease in the amount of enzyme. Fruiting also decreased porphobilinogen deaminase activity. Several kinetic constants of the C. annuum deaminase were determined.
摘要
测定了辣椒（Capsicum annuum）和一品红（Euphorbia pulcherrima）幼叶、衰老叶和成熟叶中卟胆原脱氨酶的活性。在幼叶粗提物中发现了高活性，而在衰老叶和成熟叶的提取物中几乎没有发现活性。脱氨酶活性的降低不是由于可分离抑制剂的存在。通过在 DEAE-纤维素柱上从不同年龄的叶片粗提酶提取物中进行纯化，表明脱氨酶活性的降低是由于酶量的实际减少所致。结果表明，果实形成也降低了卟胆原脱氨酶的活性。测定了 C. annuum 脱氨酶的几个动力学常数。
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