Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
Plant Physiol. 1980 Dec;66(6):1196-7. doi: 10.1104/pp.66.6.1196.
Algae of the genera Dunaliella and Asteromonas can maintain extremely high concentration gradients (>10(4)) of glycerol between the intracellular space and the medium. This unique ability is highly temperature-dependent. Treating the algae for several minutes at temperatures exceeding 60 C causes complete release of all the internally held glycerol; 50% release occurs around 50 C, but essentially none is released below 40 C. A similar behavior was observed in several species of Dunaliella, and one of Asteromonas and is independent of the salt concentration of the medium. The underlying mechanism may involve a temperature-dependent conformational transition of a component of the cellular membrane which is essential for glycerol impermeability.
杜氏藻属和节旋藻属的藻类可以在细胞内空间和培养基之间维持极高的甘油浓度梯度（>10(4)）。这种独特的能力高度依赖于温度。将藻类在超过 60°C 的温度下处理几分钟会导致所有内部甘油完全释放；在 50°C 左右释放 50%，但在 40°C 以下基本不释放。在几种杜氏藻和一种节旋藻中观察到类似的行为，并且与培养基的盐浓度无关。潜在的机制可能涉及细胞膜组件的温度依赖性构象转变，这对于甘油不可渗透性至关重要。
