ARC Research Group on Photosynthesis, Department of Botany, University of Sheffield, S10 2TN, United Kingdom.
Plant Physiol. 1981 Jan;67(1):85-8. doi: 10.1104/pp.67.1.85.
When mannose was included in the enzyme incubation medium during the preparation of protoplasts from leaves of spinach, maltose was an early product of protoplast photosynthesis and, after 12 minutes, accounted for up to 15% of the (14)C incorporated from (14)CO(2). Maltose was not detected in protoplasts prepared in the normal enzyme medium. Rapid separation of cytoplasm and chloroplasts following exposure to (14)CO(2) showed that maltose was present in both fractions. Direct measurements of [(14)C]maltose uptake indicated transport across the chloroplast envelope at rates similar to the transport of glucose. The source of maltose and site of its initial formation are discussed.
当在制备菠菜叶片原生质体的酶孵育介质中包含甘露糖时,麦芽糖是原生质体光合作用的早期产物,并且在 12 分钟后,其占从 (14)CO2 掺入的 (14)C 的高达 15%。在正常酶介质中制备的原生质体中未检测到麦芽糖。在暴露于 (14)CO2 后快速分离细胞质和叶绿体表明,麦芽糖存在于这两个部分中。对 [(14)C]麦芽糖摄取的直接测量表明,其以类似于葡萄糖的转运速率穿过叶绿体被膜进行运输。讨论了麦芽糖的来源及其初始形成的部位。