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1
Observations on the phosphate status and intracellular pH of intact cells, protoplasts and chloroplasts from photosynthetic tissue using phosphorus-31 nuclear magnetic resonance.利用磷-31核磁共振对光合组织中完整细胞、原生质体和叶绿体的磷酸盐状态及细胞内pH的观察
Biochem J. 1982 Feb 15;202(2):429-34. doi: 10.1042/bj2020429.
2
31P NMR studies of spinach leaves and their chloroplasts.菠菜叶及其叶绿体的31P核磁共振研究。
J Biol Chem. 1990 Jan 25;265(3):1319-26.
3
Inhibition of photosynthesis in isolated spinach chloroplasts by inorganic phosphate or inorganic pyrophosphatase in the presence of pyrophosphate and magnesium ions.在焦磷酸和镁离子存在的情况下，无机磷酸盐或无机焦磷酸酶对离体菠菜叶绿体光合作用的抑制作用。
Biochim Biophys Acta. 1974 Jan 18;333(1):136-40. doi: 10.1016/0005-2728(74)90169-8.
4
Formation kinetics and H2O2 distribution in chloroplasts and protoplasts of photosynthetic leaf cells of higher plants under illumination.高等植物光合作用叶片细胞叶绿体和原生质体中 H2O2 的形成动力学和分布。
Biochemistry (Mosc). 2012 Feb;77(2):143-51. doi: 10.1134/S0006297912020046.
5
Photosynthetic activities of spinach leaf protoplasts.菠菜叶原生质体的光合活性。
Plant Physiol. 1975 Apr;55(4):712-6. doi: 10.1104/pp.55.4.712.
6
Metabolite levels during induction in the chloroplast and extrachloroplast compartments of spinach protoplasts.菠菜原生质体叶绿体和叶绿体外部区域诱导过程中的代谢物水平。
Biochim Biophys Acta. 1980 Nov 5;593(1):85-102. doi: 10.1016/0005-2728(80)90010-9.
7
The distribution of metabolites between spinach chloroplasts and medium during photosynthesis in vitro.体外光合作用过程中菠菜叶绿体与培养基之间代谢物的分布
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8
Novel mannose-sequestration technique reveals variation in subcellular orthophosphate pools do not explain the effects of phosphorus nutrition on photosynthesis in Eucalyptus globulus seedlings.新型甘露糖隔离技术揭示，亚细胞正磷酸盐库的变化并不能解释磷营养对蓝桉幼苗光合作用的影响。
New Phytol. 2007;176(4):849-861. doi: 10.1111/j.1469-8137.2007.02229.x.
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Inhibition of the phosphate transporter during isolation of intact chloroplasts from leaves of sunflower.在从向日葵叶片中分离完整叶绿体的过程中抑制磷酸盐转运蛋白。
Photosynth Res. 1989 May;20(2):147-59. doi: 10.1007/BF00034123.
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Phosphorus-31 nuclear magnetic resonance analysis of internal pH during photosynthesis in the cyanobacterium Synechococcus.蓝藻聚球藻光合作用期间内部pH值的磷-31核磁共振分析
Biochemistry. 1981 Sep 29;20(20):5900-7. doi: 10.1021/bi00523a038.

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1
The Novel Coronavirus and Haemostatic Abnormalities: Pathophysiology, Clinical Manifestations, and Treatment Recommendations.新型冠状病毒与止血异常：病理生理学、临床表现与治疗推荐。
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Functional Evaluation of Proteins in Watery and Gel Saliva of Aphids.蚜虫水样唾液和凝胶状唾液中蛋白质的功能评估
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Low levels of ribosomal RNA partly account for the very high photosynthetic phosphorus-use efficiency of Proteaceae species.核糖体RNA水平较低，这在一定程度上解释了山龙眼科植物极高的光合磷利用效率。
Plant Cell Environ. 2014 Jun;37(6):1276-98. doi: 10.1111/pce.12240.
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Compartmentation and fluxes of inorganic phosphate in photosynthetic cells.光合细胞中无机磷酸盐的区室化和通量。
Planta. 1984 Nov;161(6):525-30. doi: 10.1007/BF00407084.
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A comparative study of metabolite levels in plant leaf material in the dark.黑暗条件下植物叶片物质代谢物水平的比较研究。
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The relationship between phosphate status and photosynthesis in leaves : Effects on intracellular orthophosphate distribution, photosynthesis and assimilate partitioning.叶片中磷酸盐状态与光合作用的关系：对细胞内正磷酸盐分布、光合作用和同化产物分配的影响。
Planta. 1986 Mar;167(3):369-75. doi: 10.1007/BF00391341.
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Photosynthetic characteristics of mesophyll cells isolated from cladophylls ofAsparagus officinalis L.从芦笋的营养叶中分离的叶肉细胞的光合特性。
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8
Reduced-activity mutants of phosphoglucose isomerase in the cytosol and chloroplast of Clarkia xantiana : II. Study of the mechanisms which regulate photosynthate partitioning.黄百合胞质和叶绿体磷酸葡萄糖异构酶的低活性突变体：II. 调节光合产物分配的机制研究。
Planta. 1989 May;178(1):110-22. doi: 10.1007/BF00392534.
9
Short-term water stress leads to a stimulation of sucrose synthesis by activating sucrose-phosphate synthase.短期水分胁迫通过激活蔗糖磷酸合成酶来刺激蔗糖合成。
Planta. 1989 Apr;177(4):535-46. doi: 10.1007/BF00392622.
10
Preclinical study of treatment response in HCT-116 cells and xenografts with (1) H-decoupled (31) P MRS.用（1）H 去耦（31）P MRS 对 HCT-116 细胞和异种移植物的治疗反应进行临床前研究。
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本文引用的文献

1
Accumulation of Maltose during Photosynthesis in Protoplasts Isolated from Spinach Leaves Treated with Mannose.用甘露糖处理的菠菜叶原生质体光合作用过程中麦芽糖的积累。
Plant Physiol. 1981 Jan;67(1):85-8. doi: 10.1104/pp.67.1.85.
2
Photosynthesis by isolated protoplasts, protoplast extracts, and chloroplasts of wheat: influence of orthophosphate, pyrophosphate, and adenylates.小麦分离原生质体、原生质体提取物和叶绿体的光合作用：正磷酸盐、焦磷酸盐和腺苷酸的影响
Plant Physiol. 1978 Aug;62(2):313-9. doi: 10.1104/pp.62.2.313.
3
Role of orthophosphate and other factors in the regulation of starch formation in leaves and isolated chloroplasts.正磷酸盐和其他因素在叶片和离体叶绿体淀粉形成中的作用。
Plant Physiol. 1977 Jun;59(6):1146-55. doi: 10.1104/pp.59.6.1146.
4
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.
5
A microcolorimetric method for the determination of inorganic phosphorus.一种测定无机磷的微量比色法。
J Biol Chem. 1953 Jun;202(2):675-85.
6
Localization of metabolites in animals using 31P topical magnetic resonance.使用31P局部磁共振对动物体内代谢物进行定位
Nature. 1980 Oct 23;287(5784):736-8. doi: 10.1038/287736a0.
7
A 31P-NMR study of the cross-membrane pH gradient induced by ATP hydrolysis in mitochondria.一项关于线粒体中ATP水解诱导的跨膜pH梯度的31P-核磁共振研究。
Biochim Biophys Acta. 1980 Apr 2;590(2):159-69. doi: 10.1016/0005-2728(80)90021-3.
8
Determination of intracellular pH by 31P magnetic resonance.通过31P磁共振测定细胞内pH值。
J Biol Chem. 1973 Oct 25;248(20):7276-8.

利用磷-31核磁共振对光合组织中完整细胞、原生质体和叶绿体的磷酸盐状态及细胞内pH的观察

Observations on the phosphate status and intracellular pH of intact cells, protoplasts and chloroplasts from photosynthetic tissue using phosphorus-31 nuclear magnetic resonance.

作者信息

Foyer C, Walker D, Spencer C, Mann B

出版信息

Biochem J. 1982 Feb 15;202(2):429-34. doi: 10.1042/bj2020429.

DOI:10.1042/bj2020429
PMID:7092824
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1158127/
Abstract

Individual pools of intracellular inorganic phosphate (Pi) can be observed in the dark in intact cells, protoplasts and chloroplasts from photosynthetic tissue by using 31P nuclear magnetic resonance (n.m.r.). Estimates for the pH of vacuolar and extravacuolar compartments are reported although it is shown that intracellular pH is determined by the pH of the suspending medium. Mannose treatment of asparagus (Asparagus officinalis) cells and spinach (Spinacia oleracea) protoplasts results in the inhibition of photosynthesis. The mechanism of mannose phosphate sequestration of free Pi is supported by the 31P n.m.r. spectra of mannose-treated tissue. There is a fundamental difference in 31 P n.m.r. spectra of mannose-treated spinach protoplasts and asparagus cells, reflecting a difference in the availability of vacuolar Pi for cellular metabolism in these species. The 31P n.m.r. spectrum of intact spinach chloroplasts is reported.

摘要

通过使用31P核磁共振（n.m.r.），在黑暗条件下，可以在来自光合组织的完整细胞、原生质体和叶绿体中观察到细胞内无机磷酸盐（Pi）的各个池。报告了液泡和液泡外区室pH值的估计值，尽管结果表明细胞内pH值由悬浮介质的pH值决定。甘露糖处理芦笋（Asparagus officinalis）细胞和菠菜（Spinacia oleracea）原生质体会导致光合作用受到抑制。甘露糖处理组织的31P n.m.r.光谱支持了磷酸甘露糖螯合游离Pi的机制。甘露糖处理的菠菜原生质体和芦笋细胞的31P n.m.r.光谱存在根本差异，这反映了这些物种中液泡Pi用于细胞代谢的可用性差异。报告了完整菠菜叶绿体的31P n.m.r.光谱。