Physiologie Cellulaire Végétale, Département de Recherche Fondamentale/Biologie Végétale, Centre d'Etudes Nucléaires et Université Scientifique et Médicale de Grenoble, 85X 38041 Grenoble Cedex, France.
Plant Physiol. 1981 Mar;67(3):467-9. doi: 10.1104/pp.67.3.467.
During glycine oxidation by spinach leaf mitochondria, oxygen consumption showed a strong and transient inhibition upon addition of oxaloacetate or aspartate plus alpha-ketoglutarate. During the course of the inhibition, aspartate and alpha-ketoglutarate were stoichiometrically transformed into malate and glutamate.It is concluded that oxaloacetate formed by transamination is reduced by the malate dehydrogenase, which allows the regeneration of NAD(+) for glycine oxidation and, thus, by-passes the respiratory chain. Efficiency of a malate-glutamate/aspartate-alpha-ketoglutarate shuttle upon illumination and under in vivo conditions is discussed.
在菠菜叶线粒体中甘氨酸氧化时,氧消耗在添加草酰乙酸或天冬氨酸加α-酮戊二酸时表现出强烈和短暂的抑制。在抑制过程中,天冬氨酸和α-酮戊二酸被计量转化为苹果酸和谷氨酸。因此,可以得出结论,通过转氨作用形成的草酰乙酸被苹果酸脱氢酶还原,这允许为甘氨酸氧化再生 NAD(+),从而绕过呼吸链。讨论了在光照下和体内条件下苹果酸-谷氨酸/天冬氨酸-α-酮戊二酸穿梭的效率。